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A kind of conformable biological valve and its preparation method

A bio-valve, fit-type technology, applied in the field of biomedical engineering, can solve problems such as unfavorable endothelial cell adhesion and proliferation, and affect the application effect of heart valves, so as to improve biocompatibility, maintain aesthetics, and improve mechanical properties Effect

Active Publication Date: 2022-03-11
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the fresh animal valve used in this patent has not undergone decellularization treatment, the cells existing on the surface will have a rejection reaction with the human body, which is also not conducive to the adhesion and proliferation of endothelial cells, thereby affecting the actual application effect of the heart valve.

Method used

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  • A kind of conformable biological valve and its preparation method
  • A kind of conformable biological valve and its preparation method

Examples

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Effect test

preparation example Construction

[0034] The invention provides a method for preparing a fitting biological valve, comprising the following steps:

[0035] S1. Cutting the decellularized animal valve to obtain animal valve slices 10;

[0036] S2. The polylactic acid fabric woven from the first polylactic acid yarn is fully soaked in the vascular endothelial growth factor solution, and after freeze-drying, the drug controlled release fabric 20 is obtained;

[0037]S3. Cutting the drug controlled release fabric obtained in step S2 so that its size is consistent with the animal valve slice obtained in step S1; and then attaching the two animal valve slices to the drug controlled release fabric respectively The two sides of the valve are bonded with medical bio-glue to obtain a bio-valve stent;

[0038] S4. Acupuncture the biological valve support obtained in step S3 to form a number of holes on the surface of the biological valve support;

[0039] S5. Thread the second polylactic acid yarn 30 sequentially into ...

Embodiment 1

[0049] This embodiment provides a method for preparing a conformable biological valve 100, which includes the following steps:

[0050] S1. Segmentation of animal valves

[0051] Turn on the Leica CM3050S cryostat, adjust the temperature of the sample stage to -40°C, take a fresh decellularized porcine aortic valve as the sample to be cut, blot the surface moisture with filter paper, and measure its thickness with a spiral micrometer; Place the sample to be cut on the sample stage of the cryostat, fix it with embedding agent around the sample to be cut, freeze for 10 minutes, raise the temperature to -15°C, start slicing after 5 minutes, remove the surface embedding agent, and Half of the thickness of the sample to be cut is used as the slice thickness to obtain an animal valve slice 10 with a thickness half of the original thickness of the valve, and save it for future use.

[0052] S2, preparation of drug controlled release fabric

[0053] Soak the polylactic acid fabric i...

Embodiment 2～5

[0063] Embodiments 2 to 5 respectively provide a method for preparing a conformable biological valve. Compared with Embodiment 1, the difference is that the relevant process parameters in steps S2, S4 and S5 are changed, and the remaining steps are the same as those in Embodiment 1. 1 are consistent and will not be repeated here. The parameter values ​​corresponding to each embodiment are shown in Table 1.

[0064] The technological parameter value of table 1 embodiment 2~5

[0065]

[0066] There are certain differences between the mechanical properties and the speed of promoting cell growth of the adhesive biological valves of Examples 2-5 prepared according to the above-mentioned process parameters, indicating that the growth factor concentration, the warp and weft density of the drug-controlled release fabric and the stitches can be adjusted. The needle spacing regulates the growth rate of cells, and controls the reinforcement effect of the valve by adjusting the sewin...

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Abstract

The invention provides a fitting biological valve and a preparation method thereof. In the present invention, the animal valve after decellularization is cut, and the two animal valve slices are attached to the two sides of the drug-controlled release fabric adsorbed with vascular endothelial growth factor, so that the drug-controlled release fabric is sandwiched between the two pieces. The animal valve slices are bonded by medical bio-glue to form a bio-valve stent, and then sutured with polylactic acid yarn to prepare a fitted bio-valve. Through the above method, the present invention can give full play to the synergistic effect between traditional suturing technology and induced cell growth technology, while yarn suturing enhances the mechanical properties of heart valves, growth factors induce cells to grow along the yarn to the inside of the valve, promoting biological Endothelialization of the valve, so as to obtain a conformable biological valve with high mechanical properties and high biocompatibility. Moreover, the preparation method provided by the invention has a simple process and can meet the needs of industrialized large-scale production.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a fitting biological valve and a preparation method thereof. Background technique [0002] Heart valve refers to the valve between the atrium and the ventricle or between the ventricle and the artery, which plays an irreplaceable role in the blood circulation of the heart. In each blood circulation process, the heart valve allows blood to pass through the valve opening through its own opening and closing, and prevents the blood from flowing back to the heart, thereby ensuring the normal operation of the physiological system of the human body. However, when the heart valve is damaged or pathological, the structure of the heart valve will be damaged, resulting in a series of symptoms such as fibrosis and adhesion, which in turn will affect the normal blood circulation and physiological functions. At present, heart valve-related diseases are still serious heart disea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/54A61L27/56A61L27/50A61F2/24
CPCA61L27/3625A61L27/3683A61L27/18A61L27/54A61L27/56A61L27/50A61F2/24A61L2430/20A61L2300/414A61L2300/602A61F2240/002C08L67/04
Inventor 杨红军王朝荣许愿庄燕徐卫林
Owner WUHAN TEXTILE UNIV
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