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Preparation method of three-dimensional composite porous scaffold and three-dimensional composite porous scaffold

A technology of three-dimensional composite and porous scaffolds, which is applied in the preparation of three-dimensional composite porous scaffolds. High, good cytocompatibility effect

Active Publication Date: 2021-08-03
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, a variety of preparation methods of three-dimensional porous scaffolds, including multi-layer alternating electrospinning, mechanical extrusion, template method and adding porogens, etc., but the prepared scaffolds lack interconnected pore structures and may have disadvantages such as pore-forming agent residues.

Method used

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  • Preparation method of three-dimensional composite porous scaffold and three-dimensional composite porous scaffold
  • Preparation method of three-dimensional composite porous scaffold and three-dimensional composite porous scaffold
  • Preparation method of three-dimensional composite porous scaffold and three-dimensional composite porous scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] S1: Take dichloromethane and N,N-dimethylformamide according to the volume ratio of 4:1, and mix dichloromethane and N,N-dimethylformamide evenly to obtain an organic solvent, and then follow The mass ratio of organic solvent and polycaprolactone (PCL) and cellulose nanocrystal (CNC) mass sum is that the ratio of 16% takes PCL and CNC, and PCL and CNC are stirred and mixed with organic solvent to make it dissolve, And according to the electrospinning parameters of voltage 18kV, sample injection speed 0.8mL / h, the distance between the needle and the receiver is 20cm, and the needle is 20G, electrospinning is carried out to obtain a nanofiber membrane with a diameter of 200-600nm; , the mass ratio of adding CNC to PCL is 3:100;

[0042] S2: Mix according to the volume ratio of 1:1 to obtain a mixed solvent of absolute ethanol and deionized water, then weigh 4g of the nanofiber membrane obtained in step S1 and add it to 200mL of the mixed solvent, and mechanically stir and...

Embodiment 2

[0045] S1: Take dichloromethane and N,N-dimethylformamide according to the volume ratio of 3:2, and mix dichloromethane and N,N-dimethylformamide evenly to obtain an organic solvent, and then follow The mass ratio of organic solvent and polycaprolactone (PCL) and cellulose nanocrystal (CNC) mass sum is that the ratio of 20% takes PCL and CNC, and PCL and CNC are stirred and mixed with organic solvent to make it dissolve, And according to the electrospinning parameters of voltage 18kV, sample injection speed 0.8mL / h, distance between needle and receiver 20cm, and needle 20G, electrospinning was carried out to obtain a nanofiber membrane with a diameter of 200-600nm;

[0046] S2: Mix according to the volume ratio of 1:2 to obtain a mixed solvent of absolute ethanol and deionized water, then weigh 4g of the nanofiber membrane obtained in step S1 and add it to 200mL of the mixed solvent, and mechanically stir and break it into intermittent Accelerate the stirring, the stirring spe...

Embodiment 3

[0049] In step S1, the mass ratio of the organic solvent to the sum of the mass of PCL and CNC is 14%, and other preparation steps are basically the same as in Example 1 to obtain product C.

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Abstract

The invention discloses a preparation method of a three-dimensional composite porous scaffold and the three-dimensional composite porous scaffold, and relates to the technical field of biological scaffolds. The preparation method comprises the steps: S1, dissolving polycaprolactone and cellulose nanocrystals in an organic solvent, carrying out electrostatic spinning after uniform mixing, and obtaining a nanofiber membrane; s2, mechanically stirring and crushing the nanofiber membrane in a mixed solvent, performing sieving to obtain short nanofiber filaments, and performing suction filtration to obtain a wet material; s3, dispersing the wet material in a mixed solution of absolute ethyl alcohol and deionized water, performing thermal self-aggregation reaction for 5 minutes at the temperature of 50-57 DEG C to obtain an aggregate, and performing freeze drying in a vacuum freeze dryer to obtain the three-dimensional composite porous scaffold. According to the preparation method of the three-dimensional composite porous scaffold and the three-dimensional composite porous scaffold disclosed by the invention, the composite porous scaffold is prepared by adopting a thermal self-aggregation method, and the porous scaffold with high porosity and good intercommunity is obtained.

Description

technical field [0001] The invention relates to the technical field of tissue engineering materials, in particular to a preparation method of a three-dimensional composite porous scaffold and the three-dimensional composite porous scaffold. Background technique [0002] In recent years, bone tissue engineering has developed rapidly, which provides new technical possibilities for bone defect repair. Tissue engineering is the combination of cells, materials and processing methods to prepare a scaffold that can be used as an engineered three-dimensional microenvironment for cell adhesion, proliferation, differentiation and the formation of extracellular matrix. Cooperating with appropriate biochemical and physicochemical factors, the microenvironment determined by the physical and chemical properties of the scaffold plays a vital role in cell function and subsequent tissue regeneration, thereby improving or replacing the original biological tissue. [0003] In the extracellula...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/50A61L27/18A61L27/20D04H1/728
CPCA61L27/56A61L27/50A61L27/18A61L27/20D04H1/728A61L2430/02A61L2400/12C08L67/04C08L1/02
Inventor 刘义王云鲁越
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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