Silk fibroin/silicon dioxide composite material and preparation method thereof

A technology of silk fibroin and silicon dioxide, applied in medical science, prosthesis, etc., can solve the problems of lack of solution, small thickness, low stress, etc., and achieve the effect of excellent mechanical properties, low cost, and uniform distribution

Active Publication Date: 2021-08-06
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this patent only provides a method for preparing a composite film, which is a two-dimensional material with a small thickness and low stress during the drying process. There is no corresponding solution for preparing a relatively thick three-dimensional compression-resistant composite material.
[0007] At this stage, there is no information about SF / SiO 2 A report on the related preparation method of high-strength compressive composite materials, thus preparing a SF / SiO with good mechanical properties, biocompatibility and degradability 2 Composite materials have great development prospects

Method used

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  • Silk fibroin/silicon dioxide composite material and preparation method thereof
  • Silk fibroin/silicon dioxide composite material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of the silk fibroin / silica composite material of the present embodiment specifically includes the following steps;

[0041] (1) Preparation of silk fibroin solution

[0042] Weigh 3g NaHCO 3 and 1g Na 2 CO 3 Dissolve in 4000mL deionized water, heat to boiling, put in 80g silk, keep it at 98℃ for 30min, take it out and wash it in deionized water. After repeating the above steps three times, it was placed in an oven at 60° C. to dry to obtain silk fibroin fibers.

[0043] Prepare 9.3 mol / L LiBr solution, take 100 ml of lithium bromide solution and heat it to 65°C in a water bath, add 15 g of degummed silk fibroin in several times, stir to dissolve, and continue to heat and stir for 40 min. The liquid was placed in a dialysis bag and placed in deionized water for dialysis for 72 hours, and the deionized water was replaced every 2 hours. After dialysis, the solution was filtered to obtain a silk fibroin solution. Take a small amount of solution,...

Embodiment 2

[0059] The preparation method of the silk fibroin / silica composite material of the present embodiment specifically includes the following steps;

[0060] (1) Preparation of silk fibroin solution

[0061] The preparation method of the silk fibroin solution in this example is the same as that in Example 1, and will not be repeated here.

[0062] (2) Preparation of silica sol

[0063] Add tetraethyl orthosilicate to water so that the mass ratio of tetraethyl orthosilicate and water is 1:2, stir, add silk fibroin solution, make the mass content of silk fibroin in the system 0.1%, add a small amount of Ethanol, so that the mass content of ethanol in the system is 0.5%, and hydrochloric acid is added dropwise to the system to adjust the pH value to 2, then the system is heated to 50 ° C, and the reaction is stirred for 1 hour, until the system solution is clarified and no stratification occurs, obtaining two. Silica sol.

[0064] (3) Preparation of composite solution

[0065] Th...

Embodiment 3

[0077] The preparation method of the silk fibroin / silica composite material of the present embodiment specifically includes the following steps;

[0078] (1) Preparation of silk fibroin solution

[0079] The preparation method of the silk fibroin solution in this example is the same as that in Example 1, and will not be repeated here.

[0080] (2) Preparation of silica sol

[0081] Add tetraethyl orthosilicate to water so that the mass ratio of tetraethyl orthosilicate and water is 3:4, stir, add silk fibroin solution, make the silk fibroin content in the solution 0.1%, add a small amount of ethanol , the mass content of ethanol in the solution is 0.05%, and hydrochloric acid is added dropwise to the solution to adjust the pH value to 3, then the solution is heated to 55 ° C, and the reaction is stirred for 3 hours until the solution is clarified and does not stratify to obtain silicon dioxide sol.

[0082] (3) Preparation of composite solution

[0083] The silica sol prep...

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Abstract

The invention discloses a preparation method of a silk fibroin / silicon dioxide composite material. The preparation method comprises the following steps: (1) preparing silicon dioxide sol: mixing tetraethyl orthosilicate with water, adding a silk fibroin solution, adding ethanol, adjusting the pH value of the system to 2-3, performing heating to a temperature of 50-70 DEG C, and performing a stirring reaction to obtain the silicon dioxide sol; (2) preparing a composite solution: cooling the silicon dioxide sol prepared in the step (1) to room temperature, and adding the silk fibroin solution into the silicon dioxide sol while stirring to obtain a composite solution; (3) preparing gel: pouring the composite solution into a mold, and performing reacting at 30-50 DEG C for 1-20 hours to obtain the gel; and (4) purifying, aging, drying and dehydrating the gel in the step (3) to obtain the silk fibroin / silicon dioxide composite material. According to the preparation method of the silk fibroin / silicon dioxide composite material, the prepared SF / SiO2 composite material is excellent in mechanical property and good in biocompatibility.

Description

technical field [0001] The invention relates to the technical field of organic / inorganic composite materials, in particular to a preparation method of a high-strength silk fibroin / silica composite material and a composite material prepared by the preparation method. Background technique [0002] Silk fibroin is a natural protein polymer material containing 18 amino acids such as glycine, alanine and serine. Silk fibroin has excellent biocompatibility and degradability, and has a wide range of sources and convenient preparation. It has been widely used in the field of biological materials. The use of silk fibroin to develop new tissue engineering materials has great potential for development. [0003] Despite the high strength of natural silk fibers, the regenerated silk fibroin materials have insufficient mechanical properties. Regenerated silk fibroin is often used to prepare hydrogels and protein membrane materials. It exhibits hard and brittle mechanical properties unde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/02A61L27/50A61L27/58
CPCA61L27/227A61L27/025A61L27/50A61L27/58C08L89/00
Inventor 卢神州尹祖强程康祁珍珍张子怡
Owner SUZHOU UNIV
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