A quality control detection method and standard plate of a liquid phase chip analyzer

Abstract

The invention discloses a quality control detection method and a standard plate of a liquid phase chip analyzer, wherein the method comprises the following steps: confirming that the sheath liquid bottle is filled with the sheath liquid, putting the standard plate containing the standard reagent into the analyzer; adjusting and Judging whether the standard disk is aligned with the analyzer; judging whether the sheath fluid system and cleaning system of the analyzer are normal; measuring and analyzing the fluorescence value of the standard reagent to judge whether the laser is working normally; drawing a standard curve according to the fluorescence value of the standard reagent; The fluorescence value of the standard reagent is used to calibrate the signal position of the analyzer; this method measures and analyzes the fluorescence value of the standard reagent, and uses the fluorescence value to judge whether the position of the standard disk is inserted in place, the operation of the laser, draw a standard curve, and perform signal position calibration , to effectively ensure the accuracy of the fluorescence value signal detection, so that the analyzer can display the detection results of the samples to be tested in the effective range and appropriate position during actual use, ensuring the accuracy of the experimental results.

Description

technical field [0001] The present application relates to the field of chip technology, in particular, to a quality control detection method and a standard plate of a liquid phase chip analyzer. Background technique [0002] The liquid phase chip analyzer is a high-throughput biochip detection device that integrates fluorescence-encoded microspheres, laser detection, flow cytometry, and data analysis. It can simultaneously detect up to 100 or even 500 different proteins, peptides, DNA or Target analytes such as RNA. [0003] Due to the good reproducibility of the data on the liquid chip analyzer, it is now widely used in immunology and various disease research, such as cardiovascular disease, tumor-specific marker Biomarker screening, drug therapy target development, drug therapy Effect evaluation, disease-related angiogenesis, metastasis, cell proliferation, apoptosis, inflammation, signal transduction, monitoring research of cytokine release syndrome of cell therapy, etc....

AI Technical Summary

Problems solved by technology

[0005] Existing quality control testing methods generally simply check whether each system in the analyzer can run smoothly. Under long-term use or improper storage of the analyzer, it is easy to cause pollution, which will lead to improper or even distortion of the fluorescence value display position of the test results. The operation of each system is difficult to guarantee the accuracy of the test results

[0006] For the above problems, there is no effective technical solution

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