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Method for improving biomass of chlorella based on corn starch processing wastewater

A technology for processing wastewater and cornstarch, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of reduced photosynthetic efficiency, low yield, high cost, etc., to improve light utilization and increase biomass Effect

Pending Publication Date: 2021-08-24
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of high cost and low yield in the current chlorella culture process, the present invention combines chlorella culture with corn starch processing wastewater treatment, utilizes the water resources and nutrients of wastewater, and reduces environmental pollution. At the same time, the economic benefits of chlorella cultivation are improved; at the same time, through specific light conditions, the effective photosynthetic radiation efficiency of chlorella is improved, thereby reducing the problem of photosynthetic efficiency reduction caused by turbidity, thereby increasing the biomass of chlorella

Method used

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  • Method for improving biomass of chlorella based on corn starch processing wastewater
  • Method for improving biomass of chlorella based on corn starch processing wastewater
  • Method for improving biomass of chlorella based on corn starch processing wastewater

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Experimental program
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Effect test

Embodiment 1

[0049] The regulating tank wastewater (RPW) and anaerobic wastewater (ASW) were mixed in different proportions after simple static sedimentation, and the mixing ratio of regulating tank wastewater (RPW) and anaerobic wastewater (ASW) was set to 1:0, 0 :1, 1:1, 1:2, 1:3, without sterilization, add 500ml of mixed wastewater culture solution (mixed wastewater medium) to a 1L Erlenmeyer flask, grow to logarithm in TAP medium For the chlorella in the growth stage, centrifuge at 4000rpm for 5 minutes, remove the solid in the lower layer, rinse with deionized water 2-3 times, and resuspend in different wastewater to ensure that the initial inoculation density of chlorella is 0.4±0.05g / L. Placed in a constant temperature shaker, using white light quality LEDs, the light intensity is set to 6000lux, the total light-to-dark ratio of 24h is 12h:12h, the light-to-dark ratio of one cycle within 24h is L / D=12h:12h, and the corresponding 24h cycle frequency 1 time, (that is, 12h light and 12...

Embodiment 2

[0063] Mixed wastewater contains a large number of microorganisms, which may have a certain impact on the growth of Chlorella. In order to study the influence of microorganisms in wastewater on the cultivation of Chlorella, the following experiments were carried out:

[0064] With reference to the method of embodiment 1, the difference of embodiment 2 is: the mixing ratio of regulating pond waste water (RPW) and anaerobic waste water (ASW) is set to 1:2, obtains mixed waste water culture medium;

[0065] Take the same two parts of above-mentioned mixed wastewater medium A and B;

[0066] The mixed wastewater medium A was sterilized at 120°C for 20 minutes;

[0067] Among them, the mixed wastewater medium B was not sterilized at 120°C for 20 minutes.

[0068] The chlorella growth situation in the mixed waste water culture medium of embodiment 2 through sterilization and non-sterilization treatment is as follows figure 2 shown by figure 2 It can be seen that the biomass of...

Embodiment 3

[0070] Based on the results of Examples 1 and 2, the optimal mixing ratio is RPW / ASW=1:2, without sterilization treatment. On the basis of a 24h total light-to-dark ratio of 12h:12h, set a cycle within 24h The light-to-dark ratio L / D=2h:2h, the corresponding 24h cycle frequency is 6 times, (that is, the cycle of 2h light and 2h darkness, the light-dark cycle frequency in a cycle of 24h is 6 times).

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Abstract

The invention relates to a method for improving biomass of chlorella based on corn starch processing wastewater, and belongs to the technical field of chlorella culture. According to the method, the chlorella is cultured by utilizing starch processing wastewater, and by utilizing different characteristics of wastewater in different working sections of a wastewater treatment process, regulating tank wastewater (RPW) with relatively high organic content and relatively high turbidity and anaerobic tank wastewater (ASW) with relatively low organic content and relatively low turbidity are mixed according to a certain ratio, so that a proper culture condition of the chlorella is achieved. In a chlorella culture system based on the corn starch processing wastewater, different requirements of chlorella on a light source are met by changing light and dark frequencies in a period and selecting the optimal incident wavelength, and actual problems existing in wastewater algae culture are solved by depending on a better light condition strategy.

Description

technical field [0001] The invention relates to a method for increasing the biomass of chlorella based on cornstarch processing wastewater, and belongs to the technical field of chlorella cultivation. Background technique [0002] Over the past century, due to the extensive use of fossil fuels, a series of environmental problems have been caused, such as resource shortages and the intensification of the greenhouse effect. Biodiesel is currently a widely studied biofuel. As one of the third-generation biofuel raw materials, chlorella has the advantages of fast growth rate, high photosynthetic efficiency, high oil production per unit area, and does not compete with other crops for land. [0003] In the prior art, the cultivation of Chlorella is usually carried out in a culture medium of known composition prepared artificially. The cultivation process needs to consume a large amount of raw materials such as water, nutrients and inorganic salts, and the cultivation cost is relat...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12R1/89
CPCC12N1/12C12P7/6463
Inventor 任洪艳朱国庆阮文权
Owner JIANGNAN UNIV