Application of Viniferifuran in the Preparation of Xanthine Oxidase Inhibiting Drugs
A xanthine oxidase and drug technology, applied in the directions of drug combinations, antipyretics, antibacterial drugs, etc., can solve the problems of drug production process that affects the regularity of drug use, unknown active ingredients, unsatisfactory inhibitory effect of a single component, etc. AIDS activity, good xanthine oxidase inhibitory activity, hepatoprotective effect
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Embodiment 1
[0024] Application of Viniferifuran in the preparation of xanthine oxidase inhibitory drugs, the Viniferifuran is a stilbene dimer with a benzofuran structure, and its molecular formula is C 28 h 20 o 6 , the chemical name is 5-{6-Hydroxy-2-(4-hydroxyphenyl)-4-[(Z)-2-(4-hydroxyphenyl)vinyl]-1-benzofuran-3-yl}-1,3-benzonediol ;
[0025] The structural formula of the Viniferifuran is as follows:
[0026]
[0027] The Viniferifuran is extracted from natural medicines (such as gorse root) or synthesized by using stilbene as a monomer through oxidative coupling as a key reaction.
[0028] The inhibition of xanthine oxidase is manifested by inhibiting the activity of xanthine oxidase, reducing the generation of uric acid in the body, and preventing and treating diseases related to xanthine oxidase activity such as hyperuricemia, gout, diabetic nephropathy, and cardiovascular disease. .
[0029] The xanthine oxidase inhibitory drug is made of Viniferifuran as an active ingred...
experiment example 1
[0033] XO inhibitory activity test: at 25°C, using a 96-well plate, with a total reaction volume of 200 μL, first add 50 μL of enzyme solution (the final concentration of the reaction is 0.05 U / mL), 50 μL of sample solution, and incubate at 25°C for 15 minutes. Add 50 μL of xanthine substrate (final concentration 150 μmol / L) to start the reaction. After incubating at 25°C for 20 min, 50 μL of 1 mol / L hydrochloric acid solution was added to terminate the reaction. Absorbance values were detected at 290 nm. Take the difference between this value and the OD value at the time of incubation for 0 min as the final detection result; detect the blank sample in the same way (that is, without the test drug, replace the sample solution with PBS containing 5% DMSO to determine the maximum reactivity of the enzyme) OD value, and the inhibition rate of each extract was calculated according to the following formula: inhibition rate (%)=(1-test sample average OD value / blank sample average ...
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