Culture method and application of bacillus subtilis
A technology of Bacillus subtilis and its cultivation method, which is applied in the field of cultivation of Bacillus subtilis, can solve the problems that Bacillus subtilis is easy to produce foam and the culture conditions are stable, and achieve the effects of significant living control effect, high activity, and reduced foam generation
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Embodiment 1
[0068] A method for cultivating Bacillus subtilis, comprising the following steps:
[0069] Streak inoculation of Bacillus subtilis on LB solid medium plate, and activate culture at 35°C for 18 hours to obtain the activated strain;
[0070] The activated strain was inoculated in LB liquid medium, shaken and cultured at 35°C and 180r / min for 18h to obtain a seed solution;
[0071] According to the components of the liquid seed medium: corn steeping liquid 10g / L, bean cake powder 1g / L, bran 4g / L, sodium citrate 0.1g / L, calcium chloride 0.01g / L, dipotassium hydrogen phosphate 0.01g / L, Tween 0.01g / L, prepare liquid seed culture medium, and inoculate the seed liquid that is 5% inoculum in described liquid seed culture medium, control liquid seed culture medium pH7.0-7.2; Through culture system The conditions for controlling the first fermentation are as follows: the temperature is 35°C, the time is 14h, and the dissolved oxygen is 40%; after the first fermentation is completed, t...
Embodiment 2
[0075] A method for cultivating Bacillus subtilis, comprising the following steps:
[0076] Streak inoculation of Bacillus subtilis on LB solid medium plate, and activate culture at 39°C for 28 hours to obtain the activated strain;
[0077] The activated strain was inoculated in LB liquid medium, shaken and cultivated at 39°C and 250r / min for 22h to obtain a seed solution;
[0078] According to the components of the liquid seed medium: corn steeping liquid 15g / L, bean cake powder 3g / L, bran 4g / L, sodium citrate 0.4g / L, calcium chloride 0.02g / L, dipotassium hydrogen phosphate 0.30g / L, Tween 0.03g / L, prepare liquid seed culture medium, and inoculate the seed liquid that is 8% inoculum in described liquid seed culture medium, control liquid seed culture medium pH7.0-7.2; Through culture system The conditions for controlling the first fermentation are as follows: the temperature is 38°C, the time is 20h, and the dissolved oxygen is 50%; after the first fermentation is completed,...
Embodiment 3
[0082] A method for cultivating Bacillus subtilis, comprising the following steps:
[0083] Streak inoculation of Bacillus subtilis on LB solid medium plate, and activate culture at 36°C for 20 hours to obtain the activated strain;
[0084] The activated strain was inoculated in LB liquid medium, and shaken and cultured for 20 hours under the conditions of 36°C and 200r / min to obtain the seed solution;
[0085] According to the components of the liquid seed medium: corn steeping liquid 12g / L, bean cake powder 2g / L, bran 3g / L, sodium citrate 0.3g / L, calcium chloride 0.02g / L, dipotassium hydrogen phosphate 0.05g / L, Tween 0.02g / L, prepare liquid seed culture medium, and inoculate the seed liquid that inoculum is 6% in described liquid seed culture medium, control liquid seed culture medium pH7.0-7.2; Through culture system The conditions for controlling the first fermentation are as follows: the temperature is 36°C, the time is 18h, and the dissolved oxygen is 45%; after the fi...
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