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Method for extracting vitamin K2 from fermentation liquor

A vitamin and fermented liquid technology, applied in the field of vitamin K2 preparation, can solve the problems of being unsuitable for industrial production and cumbersome process, and achieve the effects of avoiding consumption, simplifying the process, and reducing costs

Inactive Publication Date: 2021-08-27
SHANDONG RUNDE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the preparation methods of vitamin K2 mainly include chemical synthesis and microbial fermentation. Among them, using Flavobacterium to ferment and extract the metabolites in the bacteria is a common preparation method of vitamin K2. For example, some existing technologies use Using repeated freezing and thawing of Flavobacterium to break the wall and then extract the vitamin K2, but the whole process of this method is cumbersome and not suitable for industrial production and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting vitamin K2 from fermentation broth, comprising the steps of:

[0025] (1) Inoculate the Flavobacterium strain into the culture medium in the fermenter after seed cultivation. The components of the culture medium are: glycerol 7g / L, peptone 25g / L, yeast powder 3g / L, K 2 HPO 4 5g / L, NaCl 3g / L, MgSO 4 1g / L and agar 20g / L, the inoculum size of the Flavobacterium strain was 6%, the culture temperature was 25°C, the stirring speed was 250r / min, and the culture time was 6 days.

[0026] (2) After the cultivation is completed, add alum in the fermented liquid to make the thalline settle. The concentration of the sedimentation accelerator in the fermented liquid is 40 mg / L. After the thalline settles completely, filter and separate the thalline for subsequent use.

[0027] (3) Put the bacteria in a freeze dryer and quickly cool down to minus 25°C, then evacuate to 0.1mbra and start heating. After the water is sublimated, the freeze-dried bacteria are ...

Embodiment 2

[0034] A method for extracting vitamin K2 from fermentation broth, comprising the steps of:

[0035] (1) Inoculate the Flavobacterium strain into the culture medium in the fermenter after seed cultivation. The components of the culture medium are: glycerol 7g / L, peptone 25g / L, yeast powder 3g / L, K 2 HPO 4 5g / L, NaCl 3g / L, MgSO 4 1g / L and agar 20g / L, the inoculum size of the Flavobacterium strain is 6.5%, the culture temperature is 24°C, the stirring speed is 350r / min, and the culture time is 5 days.

[0036] (2) After the cultivation is completed, aluminum chloride is added in the fermented liquid to allow the thalline to settle. The concentration of the sedimentation accelerator in the fermented liquid is 60 mg / L. After the thalline has settled completely, the thalline is separated by filtration for subsequent use.

[0037] (3) Put the bacteria in a freeze dryer and quickly cool down to minus 20°C, then evacuate to 0.15mbra and start heating. After the water is sublimated...

Embodiment 3

[0044] A method for extracting vitamin K2 from fermentation broth, comprising the steps of:

[0045] (1) Inoculate the Flavobacterium strain into the culture medium in the fermenter after seed cultivation. The components of the culture medium are: glycerol 7g / L, peptone 25g / L, yeast powder 3g / L, K 2 HPO 4 5g / L, NaCl 3g / L, MgSO 4 1g / L and agar 20g / L, the inoculum size of the Flavobacterium strain was 4%, the culture temperature was 28°C, the stirring speed was 250r / min, and the culture time was 6 days.

[0046] (2) After the cultivation is completed, polyferric chloride is added in the fermented liquid to make the thallus settle. The concentration of the sedimentation accelerator in the fermented liquid is 55 mg / L. After the thalline settles completely, the thalline is separated by filtration and set aside.

[0047] (3) Put the bacteria in a freeze dryer and quickly cool down to minus 30°C, then evacuate to 0.2mbra and start heating. After the water is sublimated, the freez...

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Abstract

The invention relates to the technical field of vitamin K2 preparation, in particular to a method for extracting vitamin K2 from fermentation liquor. The method comprises the following steps: (1) inoculating flavobacterium into a culture medium for fermentation, adding a settling accelerator into the fermentation liquor after fermentation, and separating thalli for later use after the thalli in the fermentation liquor are settled; (2) freeze-drying the thalli, crushing the thalli into powder, mixing the powder with acetone, performing extraction, and taking an extract liquor for later use after the extraction is completed. (3) adding clear water into the extract liquor, and separating out precipitate after the precipitate is completely separated out so as to obtain a crude product of a vitamin K2 product. and (4) mixing the vitamin K2 crude product with acetone again for extraction, then adding clear water, and separating out a precipitate after the precipitate is completely separated out so as to obtain a vitamin K2 product. The method provided by the invention not only can effectively extract the vitamin K2 in flavobacterium, but also has the characteristics of convenience in extraction and suitability for industrial application.

Description

technical field [0001] The invention relates to the technical field of vitamin K2 preparation, in particular to a method for extracting vitamin K2 from fermentation broth. Background technique [0002] In 1943, the Danish biochemist Damm and the American scientist Doisin discovered vitamin K1 and K2. The study found that vitamin K2 played an important role in bones. In 1995, Japan began to use vitamin K2 preparations as a way to improve bone loss and pain. Drugs for porosis. With the continuous research of vitamin K2 by scientists, it is found that vitamin K2 can not only effectively prevent osteoporosis, but also supplement the protein in the body and prevent the appearance of cancer. In addition, it also has the effect of promoting The formation of prothrombin can accelerate blood coagulation, thereby ensuring normal blood coagulation, effectively helping blood and bones to maintain normal and other effects. [0003] At present, the preparation methods of vitamin K2 mainl...

Claims

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Application Information

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IPC IPC(8): C12P7/66C12N1/20C07C46/10C07C50/14C12R1/20
CPCC12P7/66C12N1/20C07C46/10C07C50/14
Inventor 卢伟张倩马善丽徐艳吴祥舟李飞
Owner SHANDONG RUNDE BIOTECH CO LTD
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