A stem section grafting method for the research on the identification signal of the rootstock and ear of melon crops

Abstract

The invention discloses a stem segment grafting method for the research on the identification signal of the rootstock and ear of melon crops, and belongs to the technical field of agricultural planting. The grafting method comprises: cultivating the melon seedling A and the melon seedling B; cutting the melon seedling A obliquely to obtain a scion with an incision, cutting the stem of the melon seedling B from the plant to obtain an in vitro rootstock with an incision; Grafting with rootstocks to obtain grafted stem segments; placing them in a healing room for healing culture; sampling the interface of the grafted stem segment, the incision of the ungrafted scion A and the ungrafted rootstock B, and then performing transcriptome sequencing to collect the differential genes of the three, respectively. The early recognition signal genes of melon stock and panicle were screened out by union analysis. The method of the invention eliminates the influence of scion leaves and rootstock roots on the signal of the grafting interface, and only retains the stem segment for grafting between tissues, which is conducive to narrowing the range of gene screening, screening out key genes for early signal recognition, and is not compatible with melon crops for grafting. Provide technical support for gender and sexuality research.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a stem segment grafting method used for research on the identification signal of rootstock and ear of melon crops. Background technique [0002] my country is a major producer and consumer of melon crops such as melons, watermelons and cucumbers, and occupies a very important position in the cultivation of melon crops in the world. The long-term fixed cultivation of land has led to serious obstacles to continuous soil cropping and the spread of soil-borne diseases, which seriously threatens the development of the industry. Grafting, as an effective solution, has now been widely used in the production of melon crops such as melons. [0003] Grafting, as a vegetative propagation technique of plants, has a history of 3,000 years of application and is now widely used in the production of vegetables, fruit trees and flowers. Grafting technology can improve ...

AI Technical Summary

Problems solved by technology

Therefore, graft affinity is an important barrier limiting the use of grafting techniques

[0005] In the prior art, Yemon and Brown (1976) proposed that there is a heterologous recognition mechanism involving cell wall polymers between rootstock cells at the grafting interface of plants; Cookson et al. (2014) analyzed the combined healing of grape autologous and heterologous grafting Gene expression at the grafting interface at different time points during the period, 1105 candidate genes related to grafting affinity were found, and the up-regulation of genes related to peroxide and disease course was found. It is believed that there is an immune recognition mechanism that affects grafting affinity; tobacco The grafting of 73 species of angiosperms from 38 families is mainly due to the fact that the β-1,4-glucanase gene (GH9B3) promotes the formation of functional tissue adhesion and promotes the co-existence between scion and rootstock. plastid transport, which solves the problem of accumulation of necrotic layers in distant grafts (Notaguchi et al 2020), however, in the tobacco family intergrafting system, where there is less reconnection of xylem and almost no reconnection of phloem, tobacco grafting does not resolve plant The problem of grafting incompatibility is that it can adhere to a variety of plants and survive, but the growth is not normal

It can be seen that the problem of low grafting affinity has not been solved in the prior art, and most of the studies on grafting affinity in the prior art use intact plants, lacking the mechanism of early signal recognition and grafting affinity in a highly controlled environment. Therefore, it is impossible to effectively solve the problem of low grafting affinity, and how to screen the early recognition signal of grafting to solve the problem of low grafting affinity has become a difficult problem to be solved by those skilled in the art.

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