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A technology for Streptococcus suis and vaccines, applied in vaccines, veterinary vaccines, antibacterial drugs, etc., can solve the problems of food safety, drug resistance, limited immune protection, etc., so as to reduce production costs and reduce vaccination workload. , reduce the effect of stress response
Active Publication Date: 2021-09-03
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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[0002] Streptococcus suis is an important zoonotic pathogen that causes serious food safety problems. Over the years, the abuse of antibiotics has led to the emergence of drug resistance, which poses a challenge to the prevention and treatment of Streptococcus suis infection
However, researchers immunized animals with a whole bacterial vaccine against S. suis and found that the whole bacteria inactivated vaccine provided limited protection against different serotypes of S. suis
[0003] In the prior art, there is a lack of Streptococcus suis disease vaccines capable of protecting Streptococcus suis Type 3 and Type 31 challenged viruses, and even more lack of Streptococcus suis disease vaccines capable of simultaneously protecting Type 2, Type 3 and Type 31 challenged viruses
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Embodiment 1
[0037] Embodiment 1 Prepares the construction of the recombinant bacterium of vaccine antigen
[0038] In order to find an effective universal vaccine antigen for Streptococcus suis, a large number of antigens were screened by immunoproteomics methods, and finally it was found that the vaccine prepared with protein A as the immunogen can protect Streptococcus suis types 2, 3 and 31 Type challenge, the gene sequence of protein A is shown in SEQ ID NO:1, and the amino acid sequence is shown in SEQ ID NO:2.
[0039] 1.PCR primers
[0040] Primers A-P1 and A-P2 were designed for amplifying the gene encoding protein A. In addition, primers B-P1 and B-P2 were designed to amplify the gene encoding the control protein, the gene sequence of the control protein is shown in SEQ ID NO:3, and the amino acid sequence is shown in SEQ ID NO:4. The specific sequences of the primers are shown in Table 1, and the primers were synthesized by Nanjing GenScript Biotechnology Co., Ltd.
[0041] T...
Embodiment 2
[0089] The immune effect of embodiment 2 protein A
[0090] 1. Active immunization of mice
[0091] The purified protein A was prepared into a vaccine by the following method: white oil 117.5mL, aluminum stearate 2.5g, mixed vigorously, shaken, autoclaved, cooled to about 50°C, shaken vigorously until transparent and homogeneous, The white oil adjuvant is obtained; take 1.08 mg / mL purified protein A aqueous solution, add protein A aqueous solution with 4% volume of sterilized Tween-80, and mix well to obtain the antigen solution. The antigen solution and the white oil adjuvant are mixed according to the volume ratio of 1:2, and emulsified to obtain the protein A vaccine.
[0092] Using the same method as above, the corresponding vaccine was prepared by replacing protein A with the control protein, which was designated as the control protein vaccine. The control protein vaccine and the protein A vaccine had the same antigen concentration.
[0093] According to the preparation ...
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Abstract
The invention provides a swine streptococcosis vaccine, and relates to the field of veterinary vaccines. According to the swine streptococcosis vaccine, an antigen is a protein with an amino acid sequence as shown in SEQ ID NO: 2. The invention also provides a preparation method of the swine streptococcosis vaccine. The preparation method comprises the following steps: mixing white oil and aluminum stearate to obtain a white oil adjuvant; adding Tween-80 into an aqueous solution of the protein with the amino acid sequence as shown in SEQ ID NO: 2, and performing uniform mixing to obtain an antigen solution; and mixing the antigen solution with the white oil adjuvant according to a volume ratio of (0.5-1.5): 2, and performing emulsifying to obtain the vaccine. Animals immunized by the swine streptococcosis vaccine can effectively resist attack of streptococcus suis type 2, type 3 and type 31, the vaccine protection rate reaches 100%, the swine streptococcosis vaccine is good in universality, the production cost and the inoculation workload are remarkably reduced, and besides, stress of the immunized animals can be reduced.
Description
technical field [0001] The invention relates to the field of veterinary vaccines, in particular to a porcine streptococcal disease vaccine. Background technique [0002] Streptococcus suis is an important zoonotic pathogen that causes serious food safety problems. Over the years, the abuse of antibiotics has led to the emergence of drug resistance, which poses a challenge to the prevention and treatment of Streptococcus suis infection. However, researchers immunized animals with a whole-bacteria vaccine against S. suis and found that the whole-bacteria inactivated vaccine provided limited protection against different serotypes of S. suis. [0003] In the prior art, there is a lack of Streptococcus suis disease vaccines capable of protecting Streptococcus suis Type 3 and Type 31 challenged viruses, and moreover, there is a lack of Streptococcus suis disease vaccines capable of simultaneously protecting Type 2, Type 3 and Type 31 challenged viruses. Contents of the invention...
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