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Method for simultaneously extracting nannochloropsis oculata protein and dietary fibers by ultrasonic assistance

A Nannochloropsis and dietary fiber technology, applied in the field of protein and dietary fiber extraction, can solve the problems of high loss rate, high protein production cost, excessive heavy metal content in dietary fiber, etc.

Pending Publication Date: 2021-09-10
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] In order to solve the problems of low utilization rate of Nannochloropsis algae residue, high production cost of Nannochloropsis dietary fiber and protein, long extraction time, high loss rate, excessive heavy metal content of obtained dietary fiber, etc., the present invention provides a A method for assisting simultaneous extraction of Nannochloropsis protein and dietary fiber, through ultrasonic-assisted alkaline extraction, to achieve the purpose of improving the extraction rate of dietary fiber and protein and shortening the extraction time; using organic acids to remove soluble dietary fiber and insoluble dietary fiber. Heavy metals, good removal effect

Method used

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  • Method for simultaneously extracting nannochloropsis oculata protein and dietary fibers by ultrasonic assistance
  • Method for simultaneously extracting nannochloropsis oculata protein and dietary fibers by ultrasonic assistance

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Embodiment 1～6

[0030] S1. Degreasing: Nannochloropsis powder is removed with supercritical CO2 to remove residual fat, extraction pressure is 20MPa, extraction temperature is 37.5°C, separation pressure is 4Mpa, separation temperature is 40°C, extraction time is 2h, and defatted Nannochloropsis powder is obtained; S2. Wall: add degreased Nannochloropsis powder to water, the mass ratio of defatted Nannochloropsis powder to water is 1:10, ultrasonically break the wall for 20 minutes; 30min; S3. Double-enzyme enzymatic hydrolysis: heat the broken defatted Nannochloropsis powder suspension to 50°C and keep it warm, add neutral protease according to the enzyme substrate mass ratio of 3%, and do not control the pH % Add cellulase, ultrasonic-assisted enzymolysis reaction for 15 minutes, the frequency of divergent ultrasonic waves used is 40kHz, and the power is 300W; Centrifugal separation at 3000rmp; S6 protein extraction: Add ammonia water to the supernatant obtained in S5 to adjust the pH to 11...

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Abstract

The invention discloses a method for simultaneously extracting a nannochloropsis oculata protein and dietary fibers by ultrasonic assistance, and relates to the technical field of extraction of a protein and dietary fibers. The method comprises the steps of degreasing, wall breaking, double-enzyme enzymolysis, enzyme deactivation, solid-liquid separation, protein extraction, soluble dietary fiber separation, heavy metal removal and the like. The method disclosed by the invention is simple to operate, high in protein and dietary fiber extraction rate and low in heavy metal content, and three products including the nannochloropsis oculata protein, the nannochloropsis oculata soluble dietary fiber and the nannochloropsis oculata insoluble dietary fiber are simultaneously obtained in one technological process.

Description

technical field [0001] The invention relates to the technical field of protein and dietary fiber extraction, in particular to a method for simultaneously extracting Nannochloropsis protein and dietary fiber with the assistance of ultrasonic waves. Background technique [0002] Nannochloropsis sp. is a single-celled marine algae with high ecological and economic value. [0003] Nannochloropsis is rich in DHA, EPA, β-carotene, etc., which can promote the development of the baby's brain and vision. It also has the effects of lowering blood fat, anti-cancer, anti-inflammation, and delaying aging. As a sustainable renewable energy source, Nannochloropsis has broad application prospects. At present, Nannochloropsis is mainly used to produce biodiesel. After oil extraction by microalgae, a large amount of algae residue will be produced, accounting for 50%-70% of the raw material. These algae residues also contain a large amount of dietary fiber and protein, accounting for 20% and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23L33/21A23L5/20A23L5/30A23L29/00
CPCA23J1/009A23L33/21A23L5/20A23L5/32A23L5/23A23L29/06A23L29/035A23L29/015A23V2002/00A23V2200/32A23V2250/5116A23V2300/14A23V2300/48A23V2300/44A23V2250/082A23V2250/022A23V2250/042A23V2250/1578A23V2250/032A23V2250/1614A23V2250/1576A23V2250/1624A23V2250/1592
Inventor 丁青芝孙年振骆琳卢歆怡任晓锋马海乐容格清程巧玉熊天成苏晓东费诗璐
Owner JIANGSU UNIV
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