Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for inducing and/or enhancing cartilage injury repair based on chemically modified mRNA (messenger ribonucleic acid) encoding protein factor

A technology of chemical modification and protein factors, applied in chemical instruments and methods, biochemical equipment and methods, botany equipment and methods, etc., can solve problems such as safety hazards, severe allergic reactions, malignant tumors, etc.

Pending Publication Date: 2021-09-10
GENEO MEDICINE CO LTD
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of adenoviruses and retroviruses in clinical gene therapy has significant safety concerns, such as the induction of severe allergic reactions and malignancies

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing and/or enhancing cartilage injury repair based on chemically modified mRNA (messenger ribonucleic acid) encoding protein factor
  • Method for inducing and/or enhancing cartilage injury repair based on chemically modified mRNA (messenger ribonucleic acid) encoding protein factor
  • Method for inducing and/or enhancing cartilage injury repair based on chemically modified mRNA (messenger ribonucleic acid) encoding protein factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The present invention will be further elaborated below in combination with the accompanying drawings and specific embodiments. The embodiments are only used to explain the present invention, and are not intended to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified.

[0024] A method for inducing and / or enhancing cartilage damage repair based on chemically modifying mRNA-encoded protein factors of the present embodiment, comprising the following steps:

[0025] Step 1. Synthesis of specific DNA template

[0026] 1. Double-stranded DNA encoding TGF-β family protein factors:

[0027] Double-stranded DNA encoding TGF-β family protein factors: gblock, directly ordered from IDT Company of the United States, the DNA sequence (995bp) information is as follows:

[0028] (1) T7 p...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of osteoarthritis, in particular to a method for inducing and / or enhancing cartilage injury repair based on a chemically modified mRNA (messenger Ribonucleic Acid) encoding protein factor. The method comprises the following steps of expressing at least one protein factor in a TGF-beta family by the chemically modified mRNA, introducing the chemically modified mRNA for coding the TGF-beta family in a local injection manner, and generating one or more protein factors for coding the TGF-beta through body cells or transfecting human bone marrow mesenchymal stem cells, further, enabling the encoding protein factor to achieve the purpose of inducing and / or enhancing cartilage injury repair. The invention provides a safe and effective method for clinical gene therapy for treating osteoarthritis, and has great significance in treating osteoarthritis.

Description

technical field [0001] The invention relates to the technical field of osteoarthritis, in particular to a method for inducing and / or enhancing cartilage damage repair based on chemically modifying mRNA-encoded protein factors. Background technique [0002] Osteoarthritis (OA) is a chronic joint disease characterized by degeneration and destruction of articular cartilage and bone hyperplasia. The degeneration of articular cartilage in osteoarthritis is arthritis caused by traumatic and degenerative osteoarthritis and rheumatoid bone. It is a serious medical problem, and it is the fourth most disabling disease in my country and the third most disabling disease in Europe and the United States. [0003] Currently, the vast majority of treatments for osteoarthritis are medications to control pain and keep swelling down. But the cartilage continues to be destroyed, unable to change the disease process, and eventually joint replacement must be performed, which is a pain point in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/495C12N15/89C12N15/12
CPCC07K14/495C07K14/51C07K14/50C12N15/89
Inventor 王刚
Owner GENEO MEDICINE CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com