Micro-fluidic chip and method for printing single particles based on step-by-step pressurization

Abstract

The invention discloses a micro-fluidic chip for printing single particles based on step-by-step pressurization and a method for printing the single particles based on step-by-step pressurization. The micro-fluidic chip for printing the single particles based on step-by-step pressurization is simple in structure, high-flux single particle printing can be achieved along with the increase of the number of capturing and printing units and the increase of the number of capturing grooves, and the printing efficiency of the single particle is improved. According to the micro-fluidic chip for printing the single particles based on step-by-step pressurization in the embodiment of the invention, automatic printing without video monitoring can be realized, equipment is simplified, manual operation is reduced, and the printing efficiency of the particles is improved. Besides, according to the method for printing the single particles based on step-by-step pressurization, capturing and printing of the particle are achieved through precise adjustment of the liquid pressure intensity, and compared with printing methods through magnetic force, sound wave force and the like in the prior art, the equipment is greatly simplified, and the cost is remarkably reduced.

Description

technical field [0001] The invention relates to the technical field of microfluidic control, and relates to a microfluidic chip and method for printing single particles based on step-by-step pressurization. Background technique [0002] Currently, single-cell analysis technology is an important tool in regenerative medicine, clinical diagnosis and cell therapy. Printed isolation of single cells seeded into individual culture chambers is key to single-cell analysis techniques. With the development of microfluidic technology, in related technologies, single cell printing methods based on microfluidic technology have been adopted, mainly: inkjet printing, single microvalve screening, double valve screening, pipette type single cell separation, etc. . Although these methods take advantage of the advantages of microfluidic technology, such as the use of less samples and reagents, fast reaction speed, a large number of parallel processing, and ready-to-use and disposable, there ...

AI Technical Summary

Problems solved by technology

[0003]The inkjet printing method has high requirements on the equipment, requires the precise cooperation of high-speed cameras and automatic auxiliary systems, and is expensive

Single micro-valve screening method and double-valve screening method, the separation process is cumbersome to operate, the chip fabrication is complicated, and multiple cells arrive at the extrusion channel at the same time, which may easily cause valve blockage, and the single-cell printing efficiency is low

The pipette-type single-cell separation method needs to rely on manual operation, and the fluid shear force is difficult to control, which is more harmful to the cells, and only one cell can be printed per suction, and the printing efficiency is low

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