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Trichoderma koningiopsis Hk37 strain, biocontrol inoculant, and preparation method of biocontrol inoculant and application of Trichoderma koningiopsis Hk37 strain and biocontrol inoculant

A technology of Trichoderma pseudocorning and biocontrol agent, which can be applied to biochemical equipment and methods, microorganism-based methods, and botanical equipment and methods, etc., and can solve problems such as increased cost and hidden dangers in food safety.

Active Publication Date: 2021-09-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the occurrence of clubroot, it is generally necessary to change the land for planting, and to use a large amount of chemical pesticides for prevention and control, which not only increases the cost greatly, but also poses hidden dangers to food safety.

Method used

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  • Trichoderma koningiopsis Hk37 strain, biocontrol inoculant, and preparation method of biocontrol inoculant and application of Trichoderma koningiopsis Hk37 strain and biocontrol inoculant
  • Trichoderma koningiopsis Hk37 strain, biocontrol inoculant, and preparation method of biocontrol inoculant and application of Trichoderma koningiopsis Hk37 strain and biocontrol inoculant
  • Trichoderma koningiopsis Hk37 strain, biocontrol inoculant, and preparation method of biocontrol inoculant and application of Trichoderma koningiopsis Hk37 strain and biocontrol inoculant

Examples

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preparation example Construction

[0041] The present invention also provides a preparation method of the above-mentioned biocontrol agent, comprising the following steps:

[0042] The above-mentioned Hk37 bacterial strain is inoculated in PDA medium, and cultivated for 7-10 days to obtain the biocontrol agent.

[0043] Unless otherwise specified, the present invention has no special requirements on the source of each component of the PDA medium, and commercially available products well known to those skilled in the art can be used.

[0044] In the present invention, in 1 L, the PDA medium preferably includes: 200 g of potatoes, 20 g of glucose, 20 g of agar and the remainder of water; the water preferably includes distilled water; the pH of the PDA medium is preferably 7.0.

[0045] In the present invention, the culture time is 7-10 days, preferably 8-9 days, more preferably 8.5 days; the culture temperature is preferably 20-30°C, more preferably 25°C.

[0046] The present invention also provides the applicat...

Embodiment 1

[0051] Isolation and identification of strains

[0052] Where and how the strain was isolated:

[0053] Collect the swollen rapeseed root tissue of the rape clubroot disease-infected field in Zhijiang City, Hubei Province, soak it in 75% ethanol (volume concentration) for 2 to 3 minutes, wash it with sterile water for 5 to 6 times, remove the root epidermis, and then cut the root Inoculate into small pieces, inoculate on PDA medium, and incubate upside down in a constant temperature culture room at 20°C for 2 to 3 days. After the tissue is covered with hyphae, use an inoculation needle to pick out mycelia in a new PDA medium, and culture for 2 ~ 3 days, repeat the purification of mycelium tips 3 ~ 4 times, obtain a fungal strain and number it as Hk37; the preparation of the PDA medium is as follows: take 200g of potatoes, 20g of glucose, 20g of agar, add distilled water to 1000mL and mix evenly , adjust the pH to 7.0, and sterilize with high pressure steam at 121° C. for 20 m...

Embodiment 2

[0062] A biocontrol agent, the preparation method of which is:

[0063] The bacterial strain purified in Example 1 was inoculated into PDA medium (the preparation method of the medium was the same as Example 1) for activation, cultivated in a constant temperature culture room at 25°C for 8 days, and used sterile ddH 2 O the conidia are washed down, and the eluate is collected to be the spore suspension of the Hk37 strain. Adjust the concentration of conidia to about 10 7 spores / mL to obtain the biocontrol agent.

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Abstract

The invention relates to the technical field of plant disease prevention and treatment, in particular to a trichoderma koningiopsis Hk37 strain, a biocontrol inoculant, a preparation method of the biocontrol inoculant and application of the trichoderma koningiopsis Hk37 strain and the biocontrol inoculant. The invention provides the Trichoderma koningii Hk37 strain, wherein the preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M2021077. The Hk37 strain provided by the invention not only has a remarkable control effect on clubroot, but also has no potential safety hazard to people and livestock; according to the embodiment, the fermentation liquor of the Hk37 strain provided by the invention can be used for remarkably inhibiting germination of resting spores of plasmodiophora brassicae, and the inhibition rate is up to 57.4%; the control effect of conidia of the strain on the clubroot of brassicaceous crop rape is as high as 57.3%, the control effect on the clubroot of brassicaceous model plant arabidopsis thaliana is as high as 68.01%, the content of plasmodiophora brassicae in host plants is remarkably reduced, and development of the plasmodiophora brassicae is inhibited.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to a Trichoderma pseudokoningii Hk37 strain, a biocontrol fungus and a preparation method and application thereof. Background technique [0002] Clubroot of cruciferous crops is a worldwide soil-borne disease caused by the infection of Plasmodiophora brassicae. It has a wide range of hosts and can harm rapeseed, Chinese cabbage, cabbage, radish, shepherd's purse and cauliflower, etc. More than 100 cruciferous crops. The disease mainly harms the root of the host, causing parenchyma cells in the root to proliferate and form tumors. In suitable years, the incidence rate of clubroot in common field plants is 20% to 50%, and it is as high as 100% in severe cases. The output of rapeseed and vegetables has been reduced by more than 20%, and in severe cases, there are even no crops. After the occurrence of clubroot, it is generally necessary to change the land...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCC12N1/14A01N63/38Y02A40/10
Inventor 陈桃赵艳丽谢甲涛姜道宏付艳苹程家森李博
Owner HUAZHONG AGRI UNIV
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