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Method for recovering hexanoic acid in biological fermentation liquor

A technology of biological fermentation and fermentation liquid, which is applied in the field of recovery of hexanoic acid in biological fermentation liquid, can solve problems such as pollution, and achieve the effects of avoiding potential safety hazards, high efficiency, and low cost

Pending Publication Date: 2021-09-10
SICHUAN JIANNANCHUN GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for reclaiming caproic acid in biological fermentation liquid, to solve the problem that in the prior art, when reclaiming caproic acid in biological fermentation liquid, using an extractant to extract caproic acid will cause the recovery of caproic acid products. Technical problems with a certain degree of exogenous pollutants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for reclaiming hexanoic acid in biological fermentation broth, comprising the steps of:

[0027] (1) Perform high-speed centrifugation on the biological fermentation liquid containing caproic acid (caproic acid content is 20g / L), and the centrifugal speed is 6500rpm / min to remove the bacteria in the fermentation liquid;

[0028] (2) Take 1000ml of biological fermentation broth treated in step (1), add yellow water to adjust the pH to 5.5;

[0029] (3) Distill directly after adjusting the pH, and connect 500ml of the fermentation product distillate. After distillation, the recovery rate of caproic acid (calculated by dividing the total amount of caproic acid recovered by the initial total amount) is 87%;

[0030] (4) The distillate was then rectified to obtain 18.3ml of hexanoic acid with a purity of 95% (detected by high performance liquid chromatography external standard method).

Embodiment 2

[0032] A method for reclaiming hexanoic acid in biological fermentation broth, comprising the steps of:

[0033] (1) Perform membrane filtration on the biological fermentation broth containing caproic acid (caproic acid content is 20g / L) with an internal pressure hollow fiber ultrafiltration membrane with a pore size of 0.22 μm to remove bacteria in the fermentation broth;

[0034] (2) Take 1000ml of biological fermentation broth treated in step (1), add tail water to adjust the pH to 5.0;

[0035] (3) Distill directly after adjusting the pH, connect 500ml of distillate, and the recovery rate of hexanoic acid after distillation is 85%;

[0036] (4) The distillate was then rectified to obtain 17.8ml of hexanoic acid with a purity of 95% (detected by the external standard method of high performance liquid chromatography).

Embodiment 3

[0038] A method for reclaiming hexanoic acid in biological fermentation broth, comprising the steps of:

[0039] (1) Perform high-speed centrifugation on the biological fermentation broth containing caproic acid (caproic acid content is 20g / L), and the centrifugal speed is 5000rpm / min to remove the bacteria in the fermentation broth;

[0040] (2) Take 1000ml of the bio-fermentation liquid treated in step (1), and add water from the brewing bottom pot to adjust the pH to 4.5;

[0041] (3) Distill directly after adjusting the pH, and receive 500ml of distillate, and the recovery rate of hexanoic acid after distillation is 92%;

[0042] (4) The distillate was then rectified to obtain 19.4ml of hexanoic acid with a purity of 95% (detected by high performance liquid chromatography external standard method).

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Abstract

The invention discloses a method for recovering hexanoic acid in biological fermentation liquor. The technical problem a certain degree of exogenous pollutants are brought to a recovered hexanoic acid product when the hexanoic acid is extracted by using an extracting agent in the prior art that when hexanoic acid in the biological fermentation liquor is recovered is solved. The method for recovering the hexanoic acid in the biological fermentation liquor comprises the following steps of (1) removing microorganisms in the biological fermentation liquor; (2) adding wine brewing wastewater until the pH value is 3.5-5.5; (3) distilling to obtain a fermented product distillate; and (4) distilling the fermentation product obtained in the step (3), rectifying and purifying to obtain the hexanoic acid. According to the method for recovering the hexanoic acid in the biological fermentation liquor, the pH of the biological fermentation liquor is adjusted by adopting the wine brewing wastewater, wastewater discharging is reduced, the purity of the recycled hexanoic acid is high, potential safety hazards are reduced, and the method is simple and convenient to operate, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to the field of brewing, in particular to a method for recovering hexanoic acid in biological fermentation liquid. Background technique [0002] The key flavor substance "ethyl caproate" in Luzhou-flavor liquor is a fermentation metabolite of pit mud microorganisms, and caproic acid is an important substrate for the synthesis of ethyl caproate, and caproic acid is one of the four major acids in solid-state fermentation liquor First, it is very important for the flavor quality of liquor. [0003] At present, for caproic acid synthesized by biological fermentation method, the recovery and extraction method of caproic acid in biological fermentation liquid mainly includes the following steps: sterilization → acid adjustment → extraction → demulsification → distillation to obtain higher purity caproic acid and recovery of extractant deal with. [0004] In the acid adjustment process, hydrochloric acid solution is used to adjust the ...

Claims

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Application Information

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IPC IPC(8): C12P7/40C07C51/44C07C53/126
CPCC12P7/40C07C51/44C07C53/126
Inventor 唐清兰邱雪刘孟华樊科权徐姿静
Owner SICHUAN JIANNANCHUN GRP CO LTD