Blood biomarker for digestive tract tumor, application and detection kit
A technology for biomarkers and gastrointestinal tumors, which is applied in the application field of assisting early diagnosis of gastrointestinal tumors, and can solve problems such as unproven biomarkers for cancer diagnosis.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0093] 1. Detection kit preparation:
[0094] 1. Preparation of standard products:
[0095] 1) Prepare mixed standard solution A:
[0096] The concentrations of 16 kinds of monosaccharides are precisely formulated as follows: mannose 1000.0mg / L, glucose 500.0mg / L, galactose 250mg / L, fucose 10.0mg / L, 1,5-anhydroglucitol 2000.0mg / L, Xylose 100.0mg / L, Rhamnose 20.0mg / L, Arabinose 20.0mg / L, Glucosamine Hydrochloride 500.0mg / L, Galactosamine Hydrochloride 500.0mg / L, Glucuronic Acid 200.0mg / L , 100.0mg / L galacturonic acid, 50.0mg / L N-acetylglucosamine, 500.0mg / L N-acetylgalactosamine, 1000.0mg / L sialic acid, 100.0mg / L iduronic acid liquid;
[0097] Prepare standard curve calibration points:
[0098] Prepare 9 standard curve calibration points (std1~std9) with blank plasma matrix (4% bovine serum albumin in PBS solution)
[0099] std9: Pipette 150 μL of mixed standard solution A and dilute to 225 μL with deionized water;
[0100] std8: Pipette 50 μL of mixed standard solution A...
Embodiment 2
[0142] 1. Experimental materials and methods
[0143] 1.1 Acquisition of blood reagents
[0144] The sampling of all blood reagents was carried out under the procedures approved by the Clinical Trial Review Committee of Zhongshan Hospital. The reagent for a cancer patient is obtained from a person diagnosed as a patient by imaging diagnostic methods and cytopathological methods.
[0145]The plasma reagents of 60 patients with liver cancer and 76 people in the control group, 38 people with colorectal cancer, 41 people with gastric cancer and 35 normal people were used for the training group sample research on the search for markers and the diagnostic ability of markers . In the later stage, 50 cases of serum reagents from liver cancer patients and 50 normal people were obtained respectively, plasma reagents from 27 colorectal cancer patients, 36 gastric cancer patients and 35 normal people were used for verification test experiments. The diagnostic ability of the experimenta...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More