Diterpenoid compound derived from Lemnalia bournei as well as preparation method and application of diterpenoid compound
A technology for diterpenoids and soft corals, which is applied in the field of diterpenoids and their preparations derived from the soft corals of Polan scaly, and can solve problems such as drugs that have not yet been seen
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Embodiment 1
[0039] From Xisha in the South China Sea Lemnalia Diterpenoids extracted from soft corals: biofloranate A−D (1−4), euplexaurene D (5) and cneorubin K (6), the chemical structure is as follows:
[0040] .
Embodiment 2
[0042] From Xisha in the South China Sea Lemnalia A preparation method for obtaining six kinds of diterpenoids by extracting and separating from soft corals, the specific steps are as follows:
[0043] 1. Frozen Nanhai Xisha Lemnalia Soft coral (wet weight 1.9 Kg) was cut into small pieces and freeze-dried (dry weight 480 g), then extracted five times with a mixture of equal volumes of dichloromethane and methanol under ultrasound, and concentrated under reduced pressure to obtain the extract 33.8 g, then extracted four times with a mixture of equal volumes of ether and water, combined the ether extracts, and concentrated under reduced pressure to obtain 25.5 g of crude extract;
[0044] 2. Dissolve the above crude extract with 20-30mL of dichloromethane first, then perform Sephadex column chromatography, using petroleum ether / dichloromethane / methanol solution with a volume ratio of 2:1:1 as the eluent The fractions were collected tube by tube, arranged according to the mole...
Embodiment 3
[0053] Compound structure identification and NMR signal assignment:
[0054] Diterpenoid 1 (Biofloranate A ): colorless oil; [α]25 D﹢34 ( c 0.1, MeOH); HRESIMS m / z 357.2400 [M + Na] + (calcd for C 21 h 34 o 3 , 357. 2508), the compound's 1 H and 13 For CNMR data see figure 1 and figure 2 , Table 1 and Table 2.
[0055] Diterpenoid 2 (Biofloranate B ): colorless oil; [α]25 D﹢33 ( c 0.1, MeOH); HRESIMS m / z 357.2395 [M + Na] + (calcd for C 21 h 34 o 3 , 357. 2508), the compound's 1 H and 13 For CNMR data see image 3 and Figure 4 , Table 1 and Table 2.
[0056] Diterpenoid 3 (Biofloranate C): colorless oil; [α]25 D-105 ( c 0.1, MeOH); HRESIMS m / z 355.2251 [M + Na] + (calcd for C 21 h 32 o 3 , 355. 2351), the compound's 1 H and 13 For CNMR data see Figure 5 and Figure 6 , Table 1 and Table 2.
[0057] Diterpenoid 4 (Biofloranate D): colorless oil; [α]25 D﹢30 ( c 0.1, MeOH); HRESIMS m / z 355.2251 [M + Na] + (calcd for C 20 h 34 O, 316. 261...
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