Heavy metal tolerant microbial strain and application thereof

A technology of heavy metals and microorganisms, applied in the field of bio-ecological restoration of heavy metal-contaminated soil, can solve the problems of low soil reuse, large engineering workload, and long time, and achieve good ecological restoration effects, low cost, and no secondary pollution Effect

Active Publication Date: 2021-09-17
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these restoration methods are costly, take a long time, require a large amount of engineering, and have a low degree of soil reuse.

Method used

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  • Heavy metal tolerant microbial strain and application thereof
  • Heavy metal tolerant microbial strain and application thereof
  • Heavy metal tolerant microbial strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of soil bacterial suspension and isolation of strains: pass the sample soil sample through a 100-mesh sieve, remove large pieces of sand, weigh 10g of soil sample, add 90mL of sterile water, shake the table for about 30min, and make the microorganisms in the soil and water The sample was mixed evenly, and the concentration of the prepared soil suspension was 0.1g / L. The soil suspension was taken in the ultra-clean workbench for concentration gradient dilution, and 1 mL of the 0.1 g / L soil suspension was drawn with a sterilized pipette tip, and added to 9 mL of in sterile water, so dilute 10 -6 .

[0021] Gradient plate configuration of heavy metal copper: Prepare 200mL LB solid medium, and then add appropriate amount of heavy metals to the prepared 100mL liquid medium, then add 1.2g agar, sterilize at 121°C for 20min. After the sterilization is completed, generally raise one side of the petri dish by about 5mm, pour about 10mL of melted medium without metal...

Embodiment 2

[0027] Effects of different formulations on the remediation effect of Pseudomonas formosana on soil heavy metals

[0028] Pseudomonas taiwanensis (Pseudomonas taiwanensis) LQCu domesticated to efficiently precipitate and solidify heavy metals was propagated in an activated medium, and kaolin, white carbon black and diatomaceous earth were used to make wettable powder (Pseudomonas taiwanensis) respectively. Quantity is 1012 / g), wettable powder sprays 1kg per mu, the result is as follows figure 2 As shown, the heavy metal concentrations of Cu, Cr, Cd, Pb and Zn water leached in soil decreased by about 10%.

[0029] Chicken manure, corn stalks and peat soil were used to prepare organic bacteria (the number of Pseudomonas taiwanensis was 1011 / g), and the application rate of organic bacteria was 5 kg per mu. The result is as image 3 As shown, after the application of the organic bacterial agent with chicken manure as the carrier, the concentration of heavy metals leached from C...

Embodiment 3

[0031] Effects of straw type and size on soil heavy metal remediation by Pseudomonas formosanum

[0032] The commonly used local straw, corn, sorghum and wheat straw were used as carriers, and the straws were crushed into lengths of 2, 5, 8 and 10 mm as carriers. The dosage is still 5kg. The result is as Figure 4 As shown, it is found that the type of straw will not affect the repair effect of the bacterial agent, and the length of the straw will affect the precipitation effect of the bacterial agent on heavy metals. Among them, the 2mm and 5mm bacterial agents are prepared, and Cu, Cr, Cd, Pb and Zn in the soil are leached by water. The concentration of heavy metals can be reduced by 75%, and the concentration of heavy metals leached from Cu, Cr, Cd, Pb and Zn in 8mm and 10mm inoculum soil can be reduced by about 63%.

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Abstract

The invention belongs to the technical field of biological-ecological restoration of heavy metal contaminated soil, and particularly relates to a heavy metal tolerant microbial strain and application thereof. The pseudomonas is preserved in China General Microbiological Culture Collection Center on April 23, 2021, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.22233, and the pseudomonas is identified as Pseudomonas formosana. The strain is screened from the soil in the Chuanquan copper tailing area, is rod-shaped, has weekly flagellum, can move, is gram-positive bacteria, and can be used for precipitation and solidification of Cu, Cr, Cd, Pb and Zn in the heavy metal contaminated soil.

Description

technical field [0001] The invention belongs to the technical field of bio-ecological restoration of heavy metal-contaminated soil, and in particular relates to a heavy metal-tolerant microbial strain and application thereof. Background technique [0002] Heavy metals are typical soil pollutants, which have the characteristics of concealment, refractory degradation, poor mobility, and easy enrichment. They can affect soil microbial flora, ecological species, and microbial processes, thereby affecting the structure and function of ecosystems. Soil heavy metal pollution is the focus and difficulty of soil ecological governance. At present, the restoration methods for soil heavy metals are mainly to reduce their mobility and bioavailability by removing or fixing pollutants. The traditional restoration methods include: engineering treatment methods based on soil and topsoil removal; ②chemical immobilization technology based on precipitation, adsorption, complexation, redox, etc....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/38
CPCB09C1/10Y02W10/10
Inventor 李君剑陈建文曹雁麟李大乐
Owner SHANXI UNIV
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