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Application based on carbon quantum dots

A technology of carbon quantum dots and inhibition, which is applied in the application field based on carbon quantum dots, can solve the problems of shock and unreported effects, and achieve the effect of inhibiting deterioration, low cost and high safety

Pending Publication Date: 2021-09-21
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the use of these drugs, there are some adverse reactions, which can cause headache, nausea, abdominal pain and even shock
Compared with traditional iron chelators, the role of carbon quantum dots as iron chelators for iron overload-related diseases has not been reported

Method used

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  • Application based on carbon quantum dots
  • Application based on carbon quantum dots
  • Application based on carbon quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Identification of bacterial samples: Take Escherichia coli and Staphylococcus aureus out of the refrigerator at 4°C, and the strain operation needs to be performed in a sterile operating table. Pick single colonies of Escherichia coli and Staphylococcus aureus and add them to liquid LB medium and In the beef extract medium, add 100 µL of bacterial solution to every 100 mL of medium, and shake the bacteria in an incubator at 37 °C and 180 rpm for about 24 h to recover. Using the plate streaking method, the bacterial liquids of Escherichia coli and Staphylococcus aureus were spread on solid LB medium and solid beef extract medium respectively, cultured in the incubator for about 20 hours, and single colonies were selected to continue amplification. Take out the bacterial liquid when the bacterial liquid is cloudy. Then, take a glass slide and drop a small drop of water on its surface, drop a small amount of bacterial solution into the water drop, drop a little crystal vio...

Embodiment 2

[0051] Embodiment 2: Embodiment 2 of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, but it is not intended as a limitation of the present invention.

[0052] Type AB wild zebrafish: Obtained from Heilongjiang Fisheries Research Institute (Chinese Academy of Fishery Sciences). Untreated zebrafish eggs (still in E3 water) were used to treat 3D cultured zebrafish embryos for drug treatment.

Embodiment 2-1

[0054] Overall Oil Red O staining

[0055] (1) Fixation: Pacts of zebrafish embryos had been placed in a six-well plate, washed 3 times with PBS, and fixed in 4% paraformaldehyde for 2h at room temperature or overnight in a 4°C refrigerator.

[0056] (2) Dehydration: First wash 3 times with 1×PBS reagent, and wash as much paraformaldehyde as possible. Infiltrate zebrafish embryos with 20%, 40%, 60%, and 80% propylene glycol solutions sequentially, and carry out the above infiltration steps for 20 minutes in a room temperature stirrer.

[0057] Staining: Use freshly prepared 0.5% red oil solution to incubate zebrafish embryos for 24 h in the dark.

[0058] (4) Cleaning: On the second day, first wash the oil red solvent with a six-well plate, then wash the oil red with 80%, 60%, 40%, and 20% propylene glycol solutions, and then wash 3 times with 1×PBS. All the above steps were carried out in a stirrer at room temperature for 20 minutes. If samples need to be preserved, zebraf...

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PUM

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Abstract

The invention relates to application carbon quantum dots, which is characterized in that the carbon quantum dot has an inhibition effect on escherichia coli, or the carbon quantum dot derived from egg white is used as an iron chelating agent to treat iron overload type non-alcoholic fatty liver disease.

Description

technical field [0001] The invention relates to an application based on carbon quantum dots. Background technique [0002] As a new type of carbon nanoparticle first invented in 2004 and named in 2006, carbon quantum dots have been continuously developed for their biomedical functions. Carbon quantum dots come from a wide range of sources, and both natural biomass and chemical organic matter can be used as raw materials for carbon quantum dots. As a natural biomass, egg white has little safety in use. Escherichia coli (E.coli) is a bacterium that can cause gastrointestinal tract infection or urinary tract and other local tissue and organ infections in humans and various animals under certain conditions. Under certain conditions, it can cause the spread or prevalence of colibacillosis. [0003] In addition, iron is one of the indispensable trace elements in the body. Iron in the circulation is combined with transferrin and transported. When the binding capacity of transfer...

Claims

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Application Information

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IPC IPC(8): A61K33/44A61P31/04A61P1/16A23L33/10A23L3/3454A61K8/19A61Q19/00
CPCA61K33/44A61P31/04A61P1/16A23L33/10A23L3/3454A61K8/19A61Q19/00A23V2002/00A61K2800/524A61K2800/10A23V2250/1576A23V2200/10A23V2200/30A23V2200/334Y02A50/30
Inventor 李丽余立冬李炳生何明月李雪婷
Owner HARBIN INST OF TECH
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