Preparation method of gas chromatographic column and chromatographic instrument device with gas chromatographic column

Abstract

The invention provides a preparation method of a gas chromatographic column and a chromatographic instrument device with the gas chromatographic column. The preparation method comprises the following steps: preparing a cyanopropyl phenyl stationary phase containing phenyl cyanopropyl with the molar ratio of 45-55% and methyl with the molar ratio of 55-45%; treating the inner wall of the elastic quartz capillary column to increase the surface roughness; dissolving the cyanopropyl phenyl stationary phase with dichloromethane to prepare a stationary phase solution, coating the inner wall of an elastic quartz capillary column with the cyanopropyl phenyl stationary phase solution by adopting a dynamic coating method, standing for a first set time length, and then blowing out the cyanopropyl phenyl stationary phase solution with inert gas such that a stationary phase film is reserved on the inner wall of the elastic quartz capillary column; and in an inert gas environment, heating the elastic quartz capillary column with the stationary phase film on the inner wall to a first set temperature, and keeping for a second set time to prepare the gas chromatographic column. The chromatograph device comprises the gas chromatographic column prepared by the method, and two ends of the gas chromatographic column are respectively connected with a sample introduction system and a detector.

Description

technical field [0001] The invention relates to the technical field of gas chromatography, in particular to a preparation method of a gas chromatography column and a chromatograph device with the chromatography column. Background technique [0002] There are many types of gas chromatographs, and their basic structures are similar. Gas chromatographs generally include gas path systems, sampling systems, separation systems, detection systems, temperature control systems and recording systems. The gas circuit system includes gas source, purification and drying tube, carrier gas flow control and gasification device. It is a closed pipeline system with continuous operation of carrier gas, which can obtain pure carrier gas with stable flow rate. Commonly used carrier gases include hydrogen, Nitrogen and argon, the purity requirement is over 99%, good chemical inertness, no reaction with related substances, ensuring air tightness, accuracy of flow measurement and stability of carri...

AI Technical Summary

Problems solved by technology

[0007] First, there is no domestic manufacturer that can produce columns for the detection of complex trans fatty acids. The chromatographic columns listed in the national standard for the detection of trans fatty acids are all foreign manufacturers. These columns are monopolized by foreign countries and are expensive.

[0008] Second, there are thousands of complex trans fatty acid isomers. It can be said that it is a worldwide problem to detect these substances. At present, the gas chromatography columns at home and abroad only solve the separation of part of the isomers. It is urgent to develop more types of fatty acid columns

[0009] Third, the inability to separate and detect trans-fatty acid isomers means that they may be eaten for a long time, and ultimately pose a huge threat to human health

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