A kind of method for preparing lecithin type n-3pufa by enzymatic method

Abstract

The invention discloses a method for preparing lecithin-type n-3 PUFA by enzymatic method, and belongs to the technical field of separation and application of enzymes. In the deep eutectic system composed of choline chloride-glycerol phosphatidylcholine, glycerol phosphatidylcholine is used as both solvent and substrate, and lipase catalyzes the esterification reaction of glycerol phosphatidylcholine and n-3PUFA, not only It can significantly improve the mass transfer of the substrate, and can absorb by-products, relieve product inhibition, and greatly increase the lecithin content and n‑3PUFA binding rate in the product. Compared with the traditional preparation of lecithin-type n-3PUFA in solvent-free system, solvent-free vacuum system and organic solvent system, the preparation of lecithin-type n-3PUFA in this system is not only safe, green and environmentally friendly, but also the lecithin in the product The content is greatly increased, and the binding rate of n-3PUFA is high; more importantly, the immobilized lipase can be used repeatedly. The method has good social, ecological and economic benefits.

Description

A kind of method for preparing lecithin type n-3PUFA by enzymatic method technical field The invention belongs to the oil processing technical field, be specifically related to a kind of method that enzymatic method prepares lecithin type n-3PUFA. Background technique [0002] PUFA (Polyunsaturated fatty acids, PUFA) refers to containing two or more double bonds and carbon Straight-chain fatty acids with a chain length of 18 to 22, usually divided into n-3 and n-6 ​​PUFAs. Compared with n-6 fatty acids, n-3PUFAs are It has attracted widespread attention due to its large health benefits. For example, as a structural component of the brain and synaptic membrane, for the heart and cardiovascular Supports health, improves vision and cognition in newborns, reduces fat accumulation, inhibits inflammatory pathways, and reduces neurodegeneration Neuroprotective effects of sexually transmitted diseases and brain aging, etc. n‑3PUFAs are usually expressed as free fatty acids,...

AI Technical Summary

Problems solved by technology

Enzymatic acid hydrolysis has the advantage of fast reaction rate, but during the reaction process, lecithin is easy to hydrolyze, the binding rate of n-3PUFA is low, n-3PUFA is easy to oxidize and the color of the product is dark, and the content of lecithin in the final product is usually lower than 20% And the binding rate of n-3PUFA is lower than 60% (Food Chem., 2014,157:132-140); enzymatic transesterification reaction rate is slower than enzymatic acid hydrolysis, and n-3PUFA is not easy to oxidize during the reaction process and the product color is relatively bright shallow, but there are still problems such as serious lecithin hydrolysis, low lecithin content in the product, and low n-3PUFA binding rate (Catal. Commun., 2016, 75:60-64); , the product has the advantages of light color and high n-3PUFA binding rate (>90%), but the reaction mass transfer is slow, it needs to be carried out under a higher vacuum degree (<400Pa) and the lecithin content in the product is low (<5%) (Food Chem., 2017, 226:165-170)

In conclusion, the current enzymatic preparation of lecithin-type n-3PUFA is difficult to achieve both high lecithin content and high n-3PUFA binding rate