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Application of urine [alpha]1-microglobulin and polypeptide fragment thereof in gestational diabetes mellitus

A technology of gestational diabetes and microglobulin, which is applied in the direction of disease diagnosis, material inspection products, measuring devices, etc., can solve the problems of frequent monitoring and achieve the effect of convenient storage

Pending Publication Date: 2021-10-12
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For patients with diagnosed gestational diabetes, the standards and requirements for blood glucose control will be stricter and the monitoring will be more frequent

Method used

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  • Application of urine [alpha]1-microglobulin and polypeptide fragment thereof in gestational diabetes mellitus
  • Application of urine [alpha]1-microglobulin and polypeptide fragment thereof in gestational diabetes mellitus
  • Application of urine [alpha]1-microglobulin and polypeptide fragment thereof in gestational diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Urine Specimen Collection and Processing

[0023] The clean midstream urine samples of 30 normal pregnant women (N) and 78 patients with clinically diagnosed gestational diabetes mellitus (GDM) were collected from the obstetrics clinic of Beijing Shijitan Hospital affiliated to Capital Medical University, aged 15-49 years. Patients with gestational diabetes mellitus (GDM group) met the 2011 American Diabetes Association (ADA) criteria for diagnosis and treatment of diabetes, and were free from acute and chronic infections, tumors, cardiovascular diseases, and severe liver and kidney dysfunction. Seventy-eight patients with gestational diabetes were divided into GM1 (FPG≤4.50mmol / l) and GM2 (FPG>4.50mmol / L) groups according to the OGTT (Oral Glucose Tolerance, OGTT) fasting plasma glucose (FPG) levels. After the urine was collected, it was centrifuged at 400×g for 5 minutes, the supernatant was taken, aliquoted, and frozen at -80°C. The clinical characteris...

Embodiment 2

[0025] Example 2 Magnetic bead purification and screening of urine peptides

[0026] Weak cation-exchange magnetic beads were used to enrich the peptides in urine, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to analyze the peptide spectrum in urine. After calibrating the instrument with a standard, mix 1 μl of the eluate with 10 μl of matrix (0.3 % α-cyano-4-hydroxycinnamic acid, HCCA), and take 1 μl to spot on the Anchorchip (Autoflex MALDITOF, Bruker-Dalton) target plate on, dry at room temperature. The sample is ionized by nitrogen laser irradiation and then subjected to mass spectrometry analysis, collecting data in the range of 1000-10000 Da, and obtaining a mass spectrogram composed of protein peaks with different mass-to-charge ratios. For each MALDI crystallization site, a total of 400 laser irradiations (50 irradiations at 8 different positions of each crystallization site), the average value represents ...

Embodiment 3

[0027] Example 3 Identification and Analysis of Differential Peptides

[0028]Take 20ul from the sample tube for LC-MS / MS analysis. EASY-nLC1000 HPLC system was used for separation. Liquid phase A was 0.1% formic acid in acetonitrile (2% acetonitrile), and liquid phase B was 0.1% formic acid in acetonitrile (98% acetonitrile). 100% solution A equilibrates a 100×100mm BEHnanoACquity column with a flow rate of 400nl / min. The chromatographic spray needle is PN: FS360-20-10-N-20-C12. Samples were separated by capillary high-performance liquid chromatography and analyzed by a Q-Exactive mass spectrometer (Thermo). The ion source voltage is 3.5kv, the analysis time is 120min, and the precursor ion scanning range is 300-2000m / z. Mass-to-charge ratios of peptides and peptide fragments were collected as follows: 20 fragments were collected after each full scan (MS2scan, HCD). At M / Z 200, MS1 has a resolution of 70,000 and MS2 has a resolution of 17,500. The data analysis softw...

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Abstract

The invention provides an application of a urine [alpha]1-microglobulin (Alpha-1-Microglobulin) and a polypeptide fragment of the urine [alpha]1-Microglobulin, and particularly relates to an application of the urine [alpha]1-Microglobulin and the polypeptide fragment of the urine [alpha]1-Microglobulin in preparation of a relevant detection preparation for gestational diabetes mellitus. Researches prove that compared with a normal gestational group, the expression of the [alpha]1-microglobulin and the polypeptide fragment thereof in a gestational diabetes mellitus group is reduced, and the [alpha]1-microglobulin and the polypeptide fragment thereof can be used for relevant detection, monitoring and auxiliary diagnosis of gestational diabetes mellitus. According to the invention, the advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of the urine sample are exerted, and the urine sample is used for detecting the urine [alpha]1-microglobulin and the polypeptide fragment thereof.

Description

technical field [0001] The present invention relates to the new application of urine α1-microglobulin and its polypeptide fragments, in particular to the application of urine α1-microglobulin and its polypeptide fragments in the preparation of relevant detection preparations for gestational diabetes. Background technique [0002] During pregnancy, in order to meet the needs of fetal growth and development, a series of changes will occur in the reproductive, blood circulation, urinary, respiratory, digestive and endocrine systems of pregnant women, especially in the endocrine system, the secretion of gonadotropins decreases, prolactin, thyroid stimulating hormone, etc. And the secretion of adrenocorticotropic hormone, glucocorticoid, aldosterone, and thyroid hormone increases. In the second and third trimesters of pregnancy, some pregnant women are less sensitive to insulin, which decomposes and antagonizes insulin and reduces insulin activity. Pregnant women cannot normally ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/042G01N2333/47
Inventor 张曼胡智颖
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
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