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Preparation method of protamine modified PLGA coated icariin nanospheres

A technology of protamine and icariin is applied in the field of preparation of PLGA-coated icariin nano-spheres, which can solve the problems of limited application, low water solubility, and it is not suitable for direct administration to act on tissues and cells. Uniform size, simple and mild preparation conditions, low cost effect

Inactive Publication Date: 2021-10-19
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, icariin has extremely low water solubility and is not suitable for direct administration to tissues and cells, thus limiting its medical application

Method used

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  • Preparation method of protamine modified PLGA coated icariin nanospheres
  • Preparation method of protamine modified PLGA coated icariin nanospheres

Examples

Experimental program
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Embodiment 1

[0019] In this embodiment, the specific preparation method steps are as follows:

[0020] Step 1: Weigh 200 mg of PLGA and dissolve in 4 mL of dichloromethane; weigh 2 mg of icariin and dissolve in 1 mL of ethanol; weigh 140 mg of PVA and dissolve in 8 mL of water; weigh 50 mg of protamine and dissolve in 10 mL of water.

[0021] Step 2: Add the icariin ethanol solution to the PLGA dichloromethane solution, stir for 15 minutes, sonicate in an ice bath for 2 minutes, add the icariin-PLGA solution dropwise to the external water phase of PVA, and stir for 15 minutes , sonicated in an ice bath for 2 minutes to form a double emulsion, stirred at room temperature for 4 hours, centrifuged at 10,000 g at 4°C for 10 minutes, and washed twice with deionized water to obtain PLGA-coated icariin nanospheres.

[0022] Step 3: resuspend the PLGA-coated icariin nanospheres obtained in step 2 in a 5 mg / mL protamine aqueous solution, stir for 12 hours, centrifuge at 10,000 g at 4°C for 10 minut...

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Abstract

The invention belongs to the technical field of biological nanomaterials, and discloses a preparation method of protamine (PS) modified PLGA coated icariin (LCA) nanospheres. The protamine modified PLGA coated icariin nanospheres (LCA at PLGA at PS) are prepared from the following raw materials according to specified parts: a dichloromethane solution of PLGA used as an oil phase; an icariin ethanol solution used as a medicine solution; a PVA aqueous solution used as an external water phase; and a protamine aqueous solution cation modified solution. The icariin ethanol solution and the PLGA dichloromethane solution are mixed to serve as the oil phase for the first time, the PVA aqueous solution serves as the water phase to react to generate PLGA coated icariin nanospheres, finally the surfaces of the nanospheres are modified with protamine for promoting cell absorptionto obtain the protamine-modified PLGA-coated icariin nanospheres, and the prepared nanospheres are uniform in size, safe and non-toxic. The invention aims to provide the preparation method of the nanospheres for improving the biological activity of icariin.

Description

technical field [0001] The invention belongs to the technical field of biological nanomaterials, and in particular relates to a preparation method of protamine-modified PLGA-coated icariin nanospheres. Background technique [0002] PLGA is a degradable high molecular weight organic compound. It is currently one of the biodegradable materials approved by the US Food and Drug Administration (FDA) for clinical treatment. It has good biocompatibility, high safety and non-toxicity. Toxicity and good encapsulation performance, it is widely used in the field of pharmacy. The negative charge on the surface of PLGA nanospheres reduces its contact with cells. Modification of PLGA nanospheres with cations can increase the interaction between nanospheres and negatively charged cell membranes, thereby promoting the cellular uptake of PLGA nanospheres. [0003] Protamine (PS) is a strong alkaline protein with good nutrition and functionality. It can lower blood pressure, suppress tumors,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K47/64A61K31/7048A61P37/02A61P19/08A61P39/06A61P31/04A61P31/10A61P35/00A61P29/00A61P31/12
CPCA61K9/5153A61K47/64A61K31/7048A61P37/02A61P19/08A61P39/06A61P31/04A61P31/10A61P35/00A61P29/00A61P31/12
Inventor 孙冬冬汪泽坤廖子彧黎雨晴王静媛杨恩东
Owner ANHUI AGRICULTURAL UNIVERSITY
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