Micro-fluidic chip and preparation and application thereof

Abstract

The invention provides a micro-fluidic chip which comprises a channel layer and a substrate layer; the channel layer comprises a sample introduction area, a mixing area and a detection area which are sequentially communicated; the mixing area comprises a plurality of mixing units; each mixing unit comprises at least one wide channel and at least one narrow channel, wherein the width of the wide channel is 100-300 microns, and the width of the narrow channel is 1 / 5-1 / 2 of the width of the wide channel; and target samples can be efficiently mixed, and functional areas including mixing, separation and detection are integrated on the chip at the same time, so that the requirements of reaction, separation, detection and the like can be met, and the production or research efficiency can be effectively improved. The obtained micro-fluidic chip can also be applied to the field of plasma lipoprotein and exosome separation, and is beneficial to promoting the application of lipoprotein and exosome in disease mechanism research and diagnosis.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a microfluidic chip. Background technique [0002] With the development of semiconductor processing technology and microfluidic technology, chemical and biological chips based on microfluidic technology continue to emerge. Microfluidic technology is based on micro-processing technology. Functional units such as micro-pipelines and micro-reactors are processed on matrix materials such as glass and high molecular polymers, so as to realize the pre-treatment and sample addition involved in the fields of biology and chemistry. , reaction, separation, analysis, cell culture and other basic operating units are integrated or basically integrated on a chip of the size of a square, to replace a technical platform for various functions of conventional chemical or biological laboratories. Specifically, the microfluidic chip significantly improves the mixing efficiency of samples by r...

AI Technical Summary

Problems solved by technology

However, due to its density and size highly overlapping with lipoproteins, and the lack of effective separation methods, plasma exosomes obtained using traditional exosome extraction and detection techniques are often mixed with a large amount of lipoproteins, which is far from meeting the requirements of high-sensitivity extraction. and requirements for testing biological samples required for liquid biopsy

The existing three-dimensional nanostructure microfluidic chip designed by the team of Professor Liu Bifeng of Huazhong University of Science and Technology, in which the array of micropillars is functionalized with intersecting multi-walled carbon nanotubes by chemical deposition, through specific recognition molecules (CD63) and unique topological nano Materials can efficiently capture exosomes, but this method is expensive and still in the stage of laboratory research, making it difficult to promote and apply it in the short term

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