A kind of functionalized gold nanocluster ratio type dopamine fluorescent probe and preparation method thereof

Abstract

The invention discloses a functionalized gold nano-cluster ratio type dopamine fluorescent probe and a preparation method thereof. The preparation method is as follows: esterification of 4-(((2,5-dioxypyrrolidin-1-yl)oxy)carbonyl phenylboronic acid and catechin is carried out, and the reaction product passes through the amino group with glutathione. The acylation reaction is modified on the surface of the gold nano-cluster, that is, the functionalized gold nano-cluster ratio-type dopamine fluorescent probe is obtained. In the functionalized gold nano-cluster ratio-type dopamine fluorescent probe prepared by the present invention, the gold nano-cluster has a fluorescence at 560 nm. The emission peak, through the introduction of a resorcinol structure into the modified ligand, will specifically react with dopamine to generate a new fluorescence emission at 461 nm, thereby forming a ratio fluorescence emission. The fluorescent probe of the present invention has good Water solubility and biocompatibility, as well as high stability, high selectivity and high sensitivity, and the preparation method is simple, the raw materials are readily available, and the yield is high, and it can be put into large-scale production.

Description

technical field [0001] The invention belongs to the technical field of fluorescent probe preparation, in particular to a functionalized gold nanocluster ratio-type dopamine fluorescent probe and a preparation method thereof. Background technique [0002] Dopamine (DA), as an important neurotransmitter in the human body, affects the human body's thinking, work, exercise and other behaviors. The abnormal content changes of dopamine indicate the occurrence of mental diseases. At present, the detection methods of dopamine mainly include electrochemical analysis, high performance liquid chromatography, capillary electrophoresis, and fluorescence spectroscopy. Although high performance liquid chromatography has obvious advantages in separation, it has high analysis cost and long analysis time. Capillary electrophoresis has short separation time and low cost, but has weak separation ability and poor reproducibility. The electrochemical analysis method is easy to operate and has hi...

AI Technical Summary

Problems solved by technology

Although high-performance liquid chromatography has obvious advantages in separation, it has high analysis cost and long analysis time. Capillary electrophoresis has short separation time and low cost, but its separation ability is weak and its reproducibility is poor.

The electrochemical analysis method is easy to operate and has high sensitivity, but has low selectivity, poor repeatability and stability

The advantages of specificity, biocompatibility, and high spatiotemporal resolution of fluorescence spectroscopy make up for the limitations of low spatiotemporal resolution and expensive instruments of other methods, but there are still limitations of aggregation fluorescence quenching that need to be resolved

Moreover, dopamine fluorescence detection methods mostly use a single fluorescence intensity as an indicator, which has certain limitations. Therefore, it is of great significance to detect dopamine sensitively and selectively.

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