Microfluidic experiment board and cell culture method

Abstract

The invention provides a microfluidic experiment board and a cell culture method. The microfluidic experiment board comprises an upper-layer runner plate, an upper-layer sealing film, a lower-layer sealing film, a porous film and a lower-layer runner plate, the upper-layer runner plate comprises a first liquid inlet, a second liquid inlet, a first liquid outlet, a second liquid outlet and an upper-layer culture cavity, the upper-layer sealing film is located below the upper-layer runner plate, the lower-layer sealing film is located below the upper-layer sealing film, the porous film is located between the upper-layer sealing film and the lower-layer sealing film, the lower-layer runner plate is located below the lower-layer sealing film and comprises a lower-layer culture chamber, the first liquid inlet, the upper-layer culture chamber and the first liquid outlet are sequentially communicated, and the second liquid inlet, the lower-layer culture chamber and the second liquid outlet are sequentially communicated. A standardized platform which can be used for single-sided cell culture or double-sided co-culture of two or more cells is provided, and comprises a culture chamber which can continuously supply liquid on two sides and can construct a physiological shear force microenvironment, so that relatively high experimental repeatability can be realized, and large-scale industrial production can be realized.

Description

technical field [0001] The invention belongs to the field of microfluidics and biopharmaceuticals, and relates to a microfluidics experiment plate and a cell culture method. Background technique [0002] Conventional cell culture is widely used as a physiological model in drug screening, new drug testing, and biological and pharmaceutical research in universities. At present, most of the cell culture methods used as physiological models for drug screening are limited to the monolayer two-dimensional culture of a single cell, and a few scientific research institutions in colleges and universities use two-dimensional or three-dimensional sphere culture of various cells as physiological models or pathological models. Model research model. However, according to the data published in various literatures in recent years, the physiological indicators presented by the two-dimensional culture of only a single cell are not the same as the biological performance of the cells in real a...

AI Technical Summary

Problems solved by technology

However, most organ chips in academia are temporarily designed and processed according to the needs of specific organ research, and do not have versatility and stability.

Moreover, due to the differences in many human operations, such as the number of cells inoculated each time, the distribution location, the experimental operation method and other human factors, the differences between the batches of the organ-on-a-chip physiological model are caused, and the repeatability of the experimental results is not good.

[0004] Bioprinting technology is a method that can help artificially construct physiological models to be standardized, quantifiable, and mass-produced while maintaining stability. Chip technology and in vitro tissue engineering have established better experimental platforms and tools. However, most conventional organ chips are not compatible with bioprinting technology, and their closed flow channels make bioprinting difficult. As a result, most of the organ chips currently on the market still use The traditional method of manually inoculating cells for experiments cannot guarantee stable and reproducible results

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