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Microalgae culture medium containing zinc ions and microalgae culture method

A technology of microalgae culture and medium, applied in the fields of bioengineering and biomass energy, can solve problems such as metabolic dysfunction, damage to the growth of microalgae, cell death, etc., to increase biomass and biomass content, and enhance antioxidant activity , the effect of overcoming the toxic effect

Active Publication Date: 2021-10-22
SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, excess Zn 2+ It can cause oxidative stress, even damage the growth of microalgae, and the accumulated reactive oxygen species (ROS) can lead to metabolic dysfunction (such as lipid peroxidation damage biomolecules, degradation of nucleic acid or protein), and even cell death

Method used

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  • Microalgae culture medium containing zinc ions and microalgae culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A method for cultivating microalgae, comprising the steps of:

[0043] S1: Prepare the medium according to the standard BG11 medium formula, then weigh an appropriate amount of zinc sulfate and dissolve it in distilled water to prepare Zn 2+ Zn with a concentration of 4mmol / L 2+ Mother liquor, add a certain volume of Zn to the medium 2+ mother liquor, so that the medium Zn 2+ The concentrations were 5 μmol / L, and the pH of the culture medium was measured with a digital display acidity meter, and the pH was adjusted to 7.1, and sterilized under high temperature and high pressure at a temperature of 115°C for 15 minutes to obtain a microalgae culture medium containing zinc ions;

[0044] S2: Centrifuge inoculate the seed solution grown to the logarithmic growth phase, resuspend and wash it once with sterile water, inoculate the seed solution at 10% (v / v) in the prepared microalgae medium containing zinc ions, and cultivate The conditions are temperature at 25°C, light ...

Embodiment 2

[0046] A method for cultivating microalgae, comprising the steps of:

[0047] S1: Prepare the medium according to the standard BG11 medium formula, then weigh an appropriate amount of zinc chloride and dissolve it in distilled water to prepare Zn 2+ Zn with a concentration of 4mmol / L 2+Mother liquor, add a certain volume of Zn to the medium 2+ mother liquor, so that the medium Zn 2+ The concentrations were 10 μmol / L, and the pH of the culture medium was measured with a digital display pH meter, adjusted to 7.1, and sterilized under high temperature and high pressure at a temperature of 115°C for 15 minutes to obtain a microalgae culture medium containing zinc ions;

[0048] S2: Centrifuge inoculate the seed solution grown to the logarithmic growth phase, resuspend and wash it once with sterile water, inoculate the seed solution at 10% (v / v) in the prepared microalgae medium containing zinc ions, and cultivate The conditions are temperature at 25°C, light intensity at 3000 l...

Embodiment 3

[0050] A method for cultivating microalgae, comprising the steps of:

[0051] S1: Prepare the medium according to the standard BG11 medium formula, then weigh an appropriate amount of zinc carbonate and dissolve it in distilled water to prepare Zn 2+ Zn with a concentration of 4mmol / L 2+ Mother liquor, add a certain volume of Zn to the medium 2+ mother liquor, so that the medium Zn 2+ The concentrations were 20 μmol / L, and the pH of the culture medium was measured with a digital pH meter, adjusted to 7.1, and sterilized under high temperature and high pressure at a temperature of 115°C for 15 minutes to obtain a microalgae culture medium containing zinc ions;

[0052] S2: Centrifuge inoculate the seed solution grown to the logarithmic growth phase, resuspend and wash it once with sterile water, inoculate the seed solution at 10% (v / v) in the prepared microalgae medium containing zinc ions, and cultivate The conditions are temperature at 25°C, light intensity at 3000 lux, an...

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Abstract

The invention discloses a microalgae culture medium containing zinc ions and a microalgae culture method. The microalgae culture medium is obtained by taking a BG11 culture medium as a basic culture medium and then adding Zn<2+> mother liquor into the basic culture medium. The method for culturing the microalgae by using the disclosed culture medium comprises the following steps: separating, purifying and pre-culturing the microalgae, preparing the culture medium containing zinc ions, inoculating and culturing, and collecting algae cells. The microalgae culture medium and the method have advantages that the growth of microalgae is stimulated by adjusting low-concentration Zn<2+>, so that more microalgae biomass is accumulated, and the contents of grease, protein and carbohydrates are increased. On the other hand, the composition of the grease is remarkably regulated, and the content of unsaturated fatty acid is remarkably increased and can reach 59% at most. According to the method, the culture process is simple and convenient, the microalgae biomass and biomass are greatly improved, the method is a more valuable microalgae culture method, and reference is provided for utilization and development of microalgae resources.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and biomass energy, and in particular relates to a microalgae culture medium containing zinc ions and a microalgae cultivation method. Background technique [0002] With the continuous development of industrialization, the consumption of fossil energy has gradually increased in the past few decades. As a photosynthetic microorganism, microalgae are widely used in fields ranging from human food and animal feed, to pharmaceuticals and biofuels, and are considered to be valuable green plants. However, the low biomass yield of microalgae makes it difficult to achieve industrialized cultivation of microalgae. It has been found that the metabolic activities of microalgae are very sensitive to changes in environmental factors, such as temperature, light intensity, and trace elements, which will affect the growth of microalgae and the content of metabolites. Among them, zinc, as an indispensable ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/38C12N1/12
Inventor 谢通慧安明哲郑佳张永奎赵前
Owner SICHUAN UNIV
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