Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of NAD+ supplement in preparation of medicine for treating acute alcoholic liver injury

A technology for alcoholic liver damage and supplements, applied in the field of medicine, can solve problems such as lack of liver damage, and achieve the effects of eliminating hangovers, preventing and hangovers, and improving alcohol tolerance

Pending Publication Date: 2021-10-29
GUANGDONG HAIHE BIOMEDICAL TECH CO LTD +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, there is a lack of effective treatments for liver damage caused by excessive alcohol consumption

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of NAD+ supplement in preparation of medicine for treating acute alcoholic liver injury
  • Application of NAD+ supplement in preparation of medicine for treating acute alcoholic liver injury
  • Application of NAD+ supplement in preparation of medicine for treating acute alcoholic liver injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Elevation of NAD+ Concentration Caused by NADH and NRH Intake

[0073] To explore whether NADH can be taken up by cells and alter NAD+ concentration, mammalian cell lines such as proinsulin cells (MIN6), neuron-like cells (LN229), muscle-like cells were treated with vehicle (control), 1mmNADH, 1mmNRH or 1mm NMN (C2C12), normal liver cells (AML12) and HEK293 cells for 3 hours.

[0074] Cells were seeded in 6-well plates until reaching approximately 80% confluency. Cells were incubated with indicated concentrations of NADH, NRH or NMN and harvested by trypsinization. NADH or NRH were treated for 3 hours unless otherwise stated. Count the number of cells by hemocytometer and trypan blue. Harvested cells were then pelleted at 3000 g for 3 min. After removing the residual medium, the preserved NAD+ was dissolved with 7% perchloric acid, and then neutralized with NaOH (2m) and K2HPO4 (500mm). The determination of intracellular NAD+ concentration is consistent w...

Embodiment 2

[0079] Low toxicity and remedial effect in the genotoxicity of embodiment 2

[0080] A sharp increase in NAD+ concentration indicates possible toxicity to cells. To assess this toxicity, C2C12, Neuro2a and AML12 cells were treated with 1 mm NADH for 24 h, and then the total cell number was measured. Specifically, C2C12, Neuro2a and AML12 cells were incubated with 1 mM NADH or NRH for 24 h and then dissociated by trypsin for cell counting. Cells were stained with trypan blue and counted with a hemocytometer. To detect apoptosis of AML12 cells, PBS, 1 mmNADH or NRH were added to 6-well plates and treated for 24 hours. Cell numbers were counted with a BD FACSCelesta flow cytometer using Annexin v / PI staining kit and Caspases3 / 7 / SYTOX dead cell staining kit (Life Technologies). Quadrants of apoptotic, necrotic, and viable cells were identified and quantified according to the manufacturer's instructions.

[0081] image 3 A is a graph of these results, data are presented as me...

Embodiment 3

[0087] Example 3 NADH in vivo experiment

[0088] In the following in vivo experiments, C57BL / 6J male mice (Jackson Memorial Laboratory) (40 days old) were used. Under standard laboratory conditions, the temperature is 22±1°C, the photoperiod is 12 / 12h, and the relative humidity is 55±5%. All animals had free access to food and water, except for designated fasting periods (and where not indicated during the 2 h fasting period prior to dosing).

[0089] In order to evaluate the biological effects of NADH on mice, C57BL / 6J mice were intraperitoneally injected with NADH 1000 mg / kg, and the content of NAD+ in tissues was measured 4 hours later. Specifically, ten 8-week-old male C57BL / 6J mice (Jackson Memorial Laboratory) were housed in polycarbonate cages with free access to food and water under a 12-h light / dark cycle. The mice were randomly divided into treatment group and control group. The treatment group was intraperitoneally injected with 1000 mg / kg NADH or NRH dissolved i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an application of an NAD+ supplement in preparation of a medicine for treating, preventing or improving acute alcoholic liver injury, and provides an application of the NAD+ supplement in preparation of a medicine for improving alcohol tolerance and an application of the NAD+ supplement in preparation of a medicine for treating, preventing or relieving alcohol hangover symptoms. Experiments prove that the effect of treating, preventing or improving the acute alcoholic liver injury is achieved by increasing the NAD+ concentration of a patient through administration of the dietary supplement and / or the pharmaceutical composition, the liver is helped to recover from liver function damage caused by harmful factors such as alcohol, hangover can be eliminated, and the hangover symptom can be treated, prevented and relieved.

Description

[0001] Cross References to Related Applications [0002] This application claims the right to PCT application PCT / US2021 / 02567 titled "METHODS OFAMELIORATING THE EFFECTS OF ALCOHOLIC LIVER DAMAGE" filed in the United States on April 2, 2021, Chinese name: "A Method for Improving Alcoholic Liver Damage" The entire content of the above-mentioned application is incorporated herein by reference. technical field [0003] The invention relates to the field of medical technology, in particular to the application of NAD+ supplements in the preparation of medicines for treating acute alcoholic liver injury. Background technique [0004] Drinking alcohol has existed as a culture for centuries. However, alcohol is also an addictive substance that can lead to abuse or binge drinking. Alcoholism caused by excessive drinking can cause many diseases and even death. [0005] Alcohol, for example, is a major cause of acute liver injury and chronic liver disease. One such disease includes...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61K31/706A61P1/16A23L33/13
CPCA61K31/7076A61K31/706A61P1/16A23L33/13A23V2002/00A23V2200/334
Inventor 吴勇周飞唐声振吴科田石榴易龙
Owner GUANGDONG HAIHE BIOMEDICAL TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com