3D-printed urethra repair and reconstruction stent and preparation method thereof
A 3D printing, urethral technology, applied in the field of medical repair and tissue engineering, to achieve ideal stability, significant mechanical properties and degradation properties, and good biocompatibility
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Embodiment 1
[0038] The preparation method of the quantum dot solution provided in this embodiment, the specific technical scheme is as follows:
[0039] The preparation method of quantum dot solution, comprises the steps:
[0040] S1, 100ml of 3-aminopropyltrimethoxysilane (APS) was added to 900ml of ultrapure water in which 20g of 1,8-naphthalimide was dissolved, and stirred for 10min to obtain a silicon quantum dot precursor solution;
[0041] S2, the silicon quantum dot precursor solution in step S1 is irradiated with ultraviolet light for 30 minutes to obtain a green fluorescent silicon quantum dot sample solution, and then cooled to below 30°C.
[0042] S3, subject the silicon quantum dot sample solution in step S2 to 6000rpm, 15min low-speed centrifugation, the purpose of low-speed centrifugation is to precipitate 1,8-naphthoimide, and the silicon quantum dots will not precipitate at this low centrifugal rate , so the resulting silicon quantum dots and APS remain in the supernatant...
Embodiment 2
[0045] The specific technical scheme of the primary culture method of adipose stem cell ADSCs provided in this embodiment is as follows:
[0046] New Zealand rabbits, 6-8 months, after general anesthesia with pentobarbital, aseptically cut 10g of fat tissue in the groin, removed the visible fascia and blood vessels, soaked in 0.25% chloramphenicol for 30 minutes, rinsed with PBS for 3 times, cut as much as possible Cut into minced meat, add 0.1% type I collagenase and digest at 37°C for 1 hour, filter through a 100-mesh filter, centrifuge at 300g for 5 minutes, resuspend in stem cell culture medium, inoculate in a 100mm cell culture dish and continue culturing, every 2 - 3 days medium change.
[0047] Composition of adipose stem cell medium: 450ml low-sugar DMEM medium + 50ml fetal bovine serum + 100U / ml penicillin + 100mg / ml streptomycin sulfate, sterilized by 0.22um filter.
Embodiment 3
[0049] The preparation of the 3D printed urethral repair and reconstruction scaffold includes the following steps:
[0050] S1, prepare bio-ink by sodium alginate SA, nano-hydroxyapatite nHA, adipose stem cell ADSCs and aqueous solution; the bio-ink includes the following components in mass percentage: sodium alginate SA is 2%; nano-hydroxyapatite nHA is 10%; ADSCs is 10% 5 individual / mL.
[0051] S2, using the bio-ink as a base material for 3D printing with a medical 3D printer to obtain a layered porous scaffold print, the 3D printing conditions are: extrusion speed 0.1ul / s, layer height 0.25mm, printing speed 180mm / min;
[0052] S3, spray the quantum dot solution on the surface of the printed part of the bracket through the electric nano sprayer, and the spraying time is 3s;
[0053] S4, spraying CaCl on the surface of the scaffold print by an electric nano-sprayer 2 solution, the spraying time is 3s, the CaCl 2 The solution is 2% CaCl 2 aqueous solution;
[0054] S5,...
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