Extraction method and extraction device of flavonoids in day lily
An extraction method and technology of an extraction device, which are applied in the field of detection and analysis, can solve problems such as poor flavonoid purity, and achieve the effects of easy absorption, saving operation time and improving extraction efficiency.
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Embodiment 1
[0030] A device for extracting flavonoids in day lily, see Figure 1-4 , including a flask 1, which is used to hold an ethanol solution with a volume fraction of 90-95% or other flavonoid extraction reagents, the flask 1 has a body and a mouth, an adapter is installed in the mouth, and the adapter An extraction device is installed on the top, and the functions of the extraction device are: firstly, placing the daylily sample, secondly, extracting flavonoids, and thirdly, refluxing the extraction liquid. A condensation pipe 2 is installed on the upper end of the extraction device, and the function of the condensation pipe 2 is to prevent volatilization of the flavonoid extraction reagent, and has the effect of condensation and reflux.
[0031] Described extraction device comprises distillation pipe 3, has sample placement chamber in described distillation pipe 3, is used to place day lily sample, and one side of described distillation pipe 3 is equipped with steam pipe 31, and ...
Embodiment 2
[0037] A device for extracting flavonoids in day lily, the structure of which is basically the same as that of Example 1, the difference is that the purifying device 6 is filled with a high-temperature-resistant adsorption material of non-flavonoid substances, such as a filter material for filtering solids, from the siphon 32 After the mixed liquid with solids in the residue flows through the purification device 6, the solids are filtered in the high-temperature resistant adsorption material, and the liquid enters the reagent reflux zone 12, and then a part of the large particle size material is blocked by the isolation device 5 , such as protein, etc. At this time, a barrier film 51 is attached in the isolating device 5, and the barrier film 51 can select a filter membrane with a molecular weight cut-off of 2000-8000. Taking the molecular weight of the extracted flavonoids as an example, if the molecular weight of the flavonoids to be extracted is 2000, then select the specifi...
Embodiment 3
[0039] A method for extracting flavonoids in day lily, comprising the following steps:
[0040] S1, drying the day lily at 40°C and pulverizing it to obtain a sample powder with a particle size of 2mm;
[0041] S2, weigh 1.00g sample powder, wrap it with filter paper, then place it in the sample placement chamber in the distillation tube 3 described in Example 1, and add 100mL of ethanol solution with a volume fraction of 95% in the flask 1, according to figure 1 The structure shown is assembled with the extraction device. The purification device 6 adopts a serpentine tube with a total length of 10 cm and an inner diameter of 1.5 cm; the flask 1 is placed in an environment of 75 ° C, and condensed water is passed into the condenser tube 2, and the reflux extraction is performed for 5 hours;
[0042] S3, after the reflux is completed, take out the purification device 6, and let it cool down to room temperature naturally. The outside of the purification device 6 is rinsed clean,...
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