Novel high-throughput closed microarray printing system
A printing system and microarray technology, applied in printing, printing machines, rotary printing machines, etc., can solve the problems of protein limited spot fixation, poor uniformity between microarray spots, cross-contamination between spots, etc., and achieve a breakthrough in the development of the bottleneck , improve uniformity, and improve the effect of protein immobilization
Pending Publication Date: 2021-10-29
PEKING UNIV
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Problems solved by technology
However, the phenomenon of coffee ring and comet tailing often appear in the microarray produced by the above technical means
Moreover, in the process of microarray production, problems such as poor uniformity between microarray spots and cross-contamination between spots will also be caused by factors such as ambient temperature, humidity, buffer viscosity, surface tension, and ionic strength.
Especially in the process of making protein microarrays, the concentration and purity of proteins can severely limit the immobilized amount of spots produced by current techniques
Method used
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[0027] 1. Fabrication of microarray printing plate: use single-side polished silicon wafer as the substrate of SU-8 mold. Preheat the wafer at 95°C for 10 min to drive off surface water and improve adhesion. After the wafer had cooled, SU-8 was added to the wafer and spun at 1300 rpm for 60 s to generate a 100 μm thick layer. The wafer was baked at 65°C for 3 min, then baked at 95°C for 2 h, and then removed to cool. A photomask was applied to the wafer, emulsion side facing SU-8, and covered with a glass plate. 430 mJ / cm using mask alignment system at 365nm 2 dose exposure. After exposure, bake at 65°C for 3 minutes, and bake at 95°C for 15 minutes to complete the crosslinking of the exposed resist. After the wafer was cooled, the wafer was immersed in PGMEA (propylene glycolmonomethyl ether acetate) for 20 min to develop, and the wafer was cleaned with isopropanol and dried in a nitrogen drying oven. After the wafer was dried, the wafer was placed in a fluorosilane (Tri...
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The invention relates to the technical field of biochips, in particular to a novel high-throughput closed microarray printing system. The system comprises a microarray printing module which comprises a microarray printing plate and a temperature control unit, and is used for printing nucleic acid, protein and the like in a microarray manufacturing solution onto the surface of a chip and meanwhile, providing an optimal adsorption temperature, a microarray printing liquid path system, and a microarray printing motion system. The microarray printing liquid path system comprises a sample adding pump, a liquid supply pump and a valve and is used for continuously adding the fixed volume of microarray manufacturing solution to the microarray printing module; and the microarray printing motion system is used for moving the microarray printing module. According to the system, the homogeneity among microarray spots can be improved, cross contamination among the spots is avoided, and particularly, the protein immobilization amount of the spots in the protein microarray is increased, so that the bottleneck of limiting the development of the biochips is broken through.
Description
technical field [0001] The invention relates to the technical field of biochips, in particular to a novel high-throughput closed microarray printing system. Background technique [0002] Biochip is a detection method that uses microarrays fabricated on a substrate to achieve specific functions, and can be applied to a wide range of fields including genomics, proteomics, lipidomics, metabolomics, and cytoomics. Because biochips have the advantages of high detection throughput, high sensitivity, less sample / reagent consumption, and convenient use, biochip-related technologies have attracted wide attention in the fields of life sciences, medicine, pharmacy, and in vitro diagnostics. As an extremely important emerging science and technology platform and a potential strategic field for national-level industrial transformation, biochips are at a very important stage of development. [0003] The manufacturing process of biochips is complicated, and the low yield rate of products i...
Claims
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Login to View More IPC IPC(8): B41F17/00C12M1/00G01N33/53
CPCB41F17/00C12M1/00G01N33/5302B41P2217/50
Inventor 王倩宋书香王静徐波许迎利闪烨朱先伟
Owner PEKING UNIV