Hydrophobic compound mass spectrometry method based on organic solvent-desorption electrospray ionization chip

A technology for desorbing electrospray and organic solvents, applied in the field of mass spectrometry detection, which can solve the problems of difficult peak identification, difficult matrix selection, low ionization efficiency, etc., to expand the detection form, improve the ionization efficiency, and accelerate the transfer of charges

Pending Publication Date: 2021-11-02
HUAZHONG NORMAL UNIV
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Problems solved by technology

Hydrophobic compounds represented by metal complexes have problems such as poor solubility and low ionization efficiency in conventional electrospray liquid systems, making it difficult to detect such compounds based on conventional chip-electrospray mass spectrometry methods
[0005] ESI-MS has problems such as poor solubility and low ionization efficiency in the analysis of hydrophobic neutral metal complexes
The important problem of MALDI-MS in the detection of metal complexes is that the interference signal generated by the organic matrix in the <500Da mass region will inhibit the signal intensity of the analyte, and the organic matrix is ​​easy to produce adducted products with the analyte, which makes it difficult to identify the peak And in some cases, it will cause false positives, and at the same time, it faces the problem of difficult matrix selection and matrix selectivity for different samples

Method used

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  • Hydrophobic compound mass spectrometry method based on organic solvent-desorption electrospray ionization chip
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  • Hydrophobic compound mass spectrometry method based on organic solvent-desorption electrospray ionization chip

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Embodiment 1

[0050] The method for mass spectrometry analysis of hydrophobic compounds based on organic solvent-desorption electrospray ionization chip is characterized in that it comprises the following steps:

[0051] (1) First introduce DMSO as a medium-weak polar organic solvent to improve the solubility of the sample;

[0052] (2) The second target compound is a liquid hydrophobic metal complex [Re(CO) 3 Cl(C 33 h 26 N 4 )]([Re-3]) structure as shown in the figure below (5 μL, 10 μg / mL), placed on the glass matrix surface of the sample carrier;

[0053] (3) Then inject the polar solvent (methanol solution containing 0.1% formic acid), medium and weak polar solvent (DMSO) and nitrogen into the corresponding channel through the syringe pump. The tip is ejected, and under the nitrogen wrap, the electrospray solution hits the sample and dissolves it, enters the ion transmission channel of the mass spectrometer, and performs mass spectrometry detection. The mass spectrometry detection ...

Embodiment 2

[0059] The method for mass spectrometry analysis of hydrophobic compounds based on organic solvent-desorption electrospray ionization chip is characterized in that it comprises the following steps:

[0060] (1) First introduce the medium and weak polar organic solvent CH 2 Cl 2 Improve the solubility of the sample;

[0061] (2) The second target compound is a solid hydrophobic metal complex [Ir-8][PF6] (sample: KBr=1:10, w / w) pressed into tablets, after infrared spectrum analysis, placed on the glass matrix of the sample carrier surface;

[0062]

[0063] (3) Polar solvent (methanol solution containing 0.1% formic acid), medium weak polar solvent (CH 2 Cl 2 ) and nitrogen into the corresponding channel, the solvent is mixed in the fish gut-shaped channel, sprayed from the electrospray tip, wrapped in nitrogen, the electrospray solution hits the sample and dissolves it, enters the ion transmission channel of the mass spectrometer, and performs mass spectrometry Detectio...

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Abstract

The invention belongs to the field of mass spectrometric detection, and particularly relates to a hydrophobic compound mass spectrometry method based on an organic solvent-desorption electrospray ionization chip. The method comprises the following steps: a mixed solvent of an organic solvent with moderate and weak polarity and a polar organic solvent is adopted, the mixed solvent is subjected to electrospray treatment and then impacts a hydrophobic compound sample, then generated sample ions to be detected enter an ion transmission channel of a mass spectrum through diffusion for mass spectrum detection, and the organic solvent with moderate and weak polarity accounts for 38 to 50% of the mixed solvent. The method can be used for mass spectrum detection of hydrophobic compounds, and ionization and detection efficiency of mass spectrum is improved. The method is suitable for samples in different forms, can detect the forms of target compounds including but not limited to liquid and solid samples and potassium bromide-containing tablet forms, and expands the sample detection forms.

Description

technical field [0001] The invention belongs to the field of mass spectrometry detection, in particular to a method for mass spectrometry analysis of hydrophobic compounds based on an organic solvent-desorption electrospray ionization chip. Background technique [0002] Mass spectrometry is an analytical method that has developed rapidly in recent years. It is based on the ionization source to achieve ionization of the components to be measured, and then separated and identified by the action of an electric field or a magnetic field. Among various types of mass spectrometry, electrospray mass spectrometry (ESI-MS) has the advantages of high sensitivity, strong qualitative ability, and good analytical reproducibility. It is widely used in the fields of biology and chemistry, and has become an important analytical technique. On the basis of it, a new method for direct and rapid analysis of environmental samples was developed: Desorption Electrospray Ionization (DESI-MS). As a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 熊博赵宇储潇然
Owner HUAZHONG NORMAL UNIV
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