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Lactobacillus paracasei YT170 and application thereof

A technology of Lactobacillus, para-cheese, applied in the field of microbiology

Pending Publication Date: 2021-11-05
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the application of Lactobacillus paracasei in animals at present.

Method used

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  • Lactobacillus paracasei YT170 and application thereof
  • Lactobacillus paracasei YT170 and application thereof
  • Lactobacillus paracasei YT170 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Screening and preservation of Lactobacillus paracasei YT170 strain.

[0041] Under sterile conditions, an appropriate amount of feces samples from Rex rabbits was taken and placed in a 1.5 mL EP tube, and 1 mL of MRS liquid medium was added to culture and activate overnight at 37°C.

[0042] Shake the culture solution well, and then carry out 10-fold gradient dilution with normal saline to obtain 10 -1 ~10 -7 Select the appropriate dilution, take 100 μL of the dilution and spread it evenly on the LAMVAB solid medium plate, and incubate at 37°C for 24-48 h.

[0043] Observe and record the characteristics of the strains, pick up suspected lactic acid bacteria colonies with an inoculation loop, repeatedly streak and separate, and culture until the colonies are purified.

[0044] Evaluate the ability of lactic acid bacteria to metabolize and utilize inulin: culture the above-mentioned isolated and purified strains in MRS liquid medium overnight, and inoculate the...

Embodiment 2

[0049] Example 2: Inulin metabolism utilization characteristics of Lactobacillus paracasei YT170.

[0050] Growth curve and pH measurement: Streak the preserved Lactobacillus paracasei YT170 on the MRS plate, culture at 37°C for 24-48 hours, pick a single colony in the MRS liquid medium, and culture solution (1%, v / v) Inoculate in cells containing 50 mL bMRS+inulin, bMRS+glucose and bMRS+H 2 O in a sterile centrifuge tube, cultured at 37°C, and collected at 14 different time points including 1, 2, 3, 4, 6, 8, 10, 12, 14, 15, 20, 24, 48 and 72 h. The fermentation broth was passed through a spectrophotometer at a wavelength of 600 nm (OD 600 nm ) to measure the growth of lactic acid bacteria, and use a pH meter to measure the pH value of the fermentation broth.

[0051] Thin-layer chromatography (TLC) analysis of metabolites: Use TLC to analyze the dynamic changes of the inulin composition of Lactobacillus in the process of fermenting inulin, and centrifuge the fermentation br...

Embodiment 3

[0056] Example three: identification of bacterial strains.

[0057] Morphological characteristics: The colony morphology of Lactobacillus paracasei YT170 is as follows Figure 5 As shown, the colony is milky white, round and flat, with white dots in the center, and the surface is moist.

[0058] Gram staining: use the Gram staining method to detect the characteristics of the strain of the present invention. The result is as Figure 6 As shown, the bacterial strain of the present invention is a Gram-positive bacterium, and the cell morphology is rod-shaped without spores, which is a typical feature of Lactobacillus.

[0059] 16S rRNA identification: Genomic DNA of lactic acid bacteria was extracted using Bacterial Genomic DNA Extraction Kit DP302 (Tiangen Biochemical Technology Co., Ltd., Beijing, China).

[0060] Bacterial 16S rRNA gene universal primers: 27F-5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R-5'-GGTTACCTTGTTACGACTT-3' (Shanghai Sangon Biotechnology Co., Ltd., Shanghai, C...

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Abstract

The invention discloses lactobacillus paracasei YT170 and an application thereof, and belongs to the field of microorganisms. The preservation number of the lactobacillus paracasei YT170is CGMCC No.22089, the lactobacillus paracasei YT170is preserved in the China General Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.1 Yard, Beichen West Road, Chaoyang District, Beijing. The strain provided by the invention is obtained by screening and separating from rex rabbit manure, and has the capability of efficiently metabolizing and utilizing inulin oligosaccharides. The invention also provides a related application of the lactobacillus paracasei YT170. A microecological preparation or a lactobacillus composition prepared from the strain can be widely applied to animal breeding industry as a feed, an additive thereof, milk replacer and other products, so that the death rate is reduced, the use of antibiotics is reduced, and increasing the economic benefit of the breeding industry is facilitated. In addition, the method is also suitable for preparing products such as food, health-care products and medicines.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Lactobacillus paracasei and application thereof. Background technique [0002] In recent years, due to the rapid economic development and the improvement of material living standards, people's demand for animal meat products has greatly increased. In order to meet market demand, many breeding industries have gradually scaled up. However, large-scale farmed animals, especially during the weaning period, often suffer from diarrhea, which seriously affects the development of the breeding industry. For example, according to the statistics of the epidemic situation of rabbits, among the rabbits with an average mortality rate of 24%, more than 75% of the rabbits are caused by diarrhea. In order to improve the breeding rate, antibiotics are often widely used in the prevention and treatment of diseases and as antibacterial growth promoters. However, long-term use of antibiotics will lead...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/04A23K10/18A23C11/00A23K30/18A23K50/50A23K20/142A23K20/174A23K20/20A23K20/22A23K20/147A23K20/158A23K20/163A23L33/135A61K35/747A61P1/00C12R1/225A61K31/733
CPCC12N11/04A23K10/18A23C11/00A23K30/18A23K50/50A23K20/142A23K20/174A23K20/20A23K20/22A23K20/147A23K20/158A23K20/163A23L33/135A61K35/747A61K31/733A61P1/00A23V2002/00A23V2400/165A23V2200/30A61K2300/00
Inventor 祝元婷郭海英周君李蕊彤左勇
Owner SICHUAN NORMAL UNIVERSITY
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