Method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules

A technology of traditional Chinese medicine formula granules and ginger, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problem that the identification of ginger is still blank and the like.

Active Publication Date: 2021-11-09
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the identification of ginger in Jiangzhuru traditional Chinese medicine formula granules is still blank.

Method used

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  • Method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules
  • Method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules
  • Method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules

Examples

Experimental program
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Effect test

Embodiment 1

[0090] This embodiment provides a method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules, mainly using thin-layer chromatography for identification, including the following steps:

[0091] 1. Instruments, reagents and reagents

[0092] Instruments: TLC automatic imager (CAMAG REPROSTAR 3, Swiss Cama company), fully automatic thin layer spotting instrument (AUTOMATIC TLC SAMPLER4, Swiss Cama company), 1 / 10,000 balance (ME204E, Swiss Mettler), Ultrasonic cleaner (KQ-500DE, Kunshan Ultrasonic Instrument Co., Ltd.), ultrapure water system (Milli-Q Direct, Merck Co., Ltd.), water bath (HWS-28, Shanghai Yiheng Technology Co., Ltd.); double tank Expansion cylinder; silica gel G thin-layer plate (G, batch number: 20191008, Qingdao Ocean Chemical Co., Ltd.), silica gel G thin-layer plate (G, batch number: 20210326, Qingdao Puke Separation Material Co., Ltd.), silica gel G thin-layer plate (TLC Silica gel 60, batch number: HXD4333226, Merck Co., Ltd.)...

Embodiment 2

[0128] The present embodiment provides a method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules, comprising the following steps:

[0129] 1 Preparation of the test solution

[0130] Take about 2.5g of Jiangzhuru traditional Chinese medicine formula granules (CG2006002, CG2006003, CG2006004), grind them finely, add 50mL of 80% methanol, ultrasonicate for 30 minutes, let cool, filter, evaporate the filtrate to dryness, add 20mL of water to the residue, and dissolve them with ethyl acetate Shake and extract the ester twice, 20 mL each time, combine the ethyl acetate solution, evaporate to dryness, add 2 mL of methanol to the residue to dissolve, add to a polyamide column (polyamide 100 mesh to 200 mesh, 2 g, inner diameter 2 cm, dry packing Column), elute with 50mL of 30% methanol, collect the eluent, evaporate to dryness, add 1mL of methanol to the residue to dissolve, and use it as the test solution.

[0131] 2 Preparation of reference substa...

Embodiment 3

[0140] The present embodiment is a variation example of embodiment 2, and the variation with respect to embodiment 2 mainly includes the steps of "preparation of 1 need testing solution", specifically including:

[0141] Take about 2.5g of Jiangzhuru traditional Chinese medicine formula granules (CG2006002, CG2006003, CG2006004), grind them finely, add 50mL of 78% methanol, treat with ultrasound for 30 minutes, let cool, filter, evaporate the filtrate to dryness, add 20mL of water to the residue to dissolve, and use ethyl acetate Shake and extract the ester once, 15 mL each time, combine the ethyl acetate solution, evaporate to dryness, add 2 mL of methanol to the residue to dissolve, add to a polyamide column (polyamide 100-200 mesh, 1.5 g, inner diameter 1.5 cm, dry 50mL of 25% methanol was used for elution, the eluate was collected, evaporated to dryness, and the residue was dissolved in 1mL of methanol as the test solution.

[0142] See the test results Figure 12 : In th...

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Abstract

The invention relates to a method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules. The identification method comprises the following steps: providing a reference substance solution, preparing a test solution, and detecting the reference substance solution and the test solution by adopting a thin layer chromatography, wherein a reference substance in the reference substance solution contains zingiberone and 6-gingerol; the step of preparing the test solution comprises the following steps: preparing an extracting solution containing zingiberone and 6-gingerol from the ginger and bamboo shavings traditional Chinese medicine formula granules; then adopting a polyamide column to perform column chromatography on the extracting solution containing zingerone and 6-gingerol, wherein the conditions of column chromatography are as follows: the polyamide column is adopted as a chromatographic column, an eluent is a methanol solution with the methanol volume percent of 25%-55%, and isocratic elution is adopted as an elution mode. According to the method, the ginger in the Jiangzhuru traditional Chinese medicine formula granules is identified based on the thin-layer chromatography, and a scientific, rapid and low-cost new method is provided for quality control of Jiangzhuru traditional Chinese medicine formula granule products.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for identifying ginger in Jiangzhuru traditional Chinese medicine formula granules. Background technique [0002] Jiangzhuru traditional Chinese medicine formula granules are obtained by extracting Jiangzhuru decoction pieces with water, concentrating and drying them. Ginger bamboo rut decoction pieces are processed from bamboo rut, which is roasted or fried with ginger juice until yellow. It is shaped like a bamboo rut, with a yellow surface and a slight ginger aroma. Jiang Zhuru is good at resolving phlegm and relieving vomiting. [0003] Jiangzhuru traditional Chinese medicine formula granules, the problems faced in its identification include: traditional Chinese medicine formula granules are granules made of single traditional Chinese medicine decoction pieces by water extraction, separation, concentration, drying and granulation. Tra...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/94
CPCG01N30/90G01N30/94Y02A50/30
Inventor 吕渭升潘晓君杨文惠侯栩轩叶梅霞李振雨何民友陈向东孙冬梅魏梅
Owner GUANGDONG YIFANG PHARMA
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