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Burkholderia gladioli A1-07 and application thereof

A technology of Burkholderia Populus and Holderella, which is applied in the field of microorganisms, can solve problems such as large acid-base wastewater, and achieve the effects of high production efficiency, good industrial application prospects and easy operation.

Active Publication Date: 2021-11-19
SHAANXI HEALTHFUL BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method produces a large amount of acid-base wastewater, which puts great pressure on environmental protection treatment.

Method used

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  • Burkholderia gladioli A1-07 and application thereof
  • Burkholderia gladioli A1-07 and application thereof
  • Burkholderia gladioli A1-07 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] Embodiment 1: the present invention prepares ferulic acid by oryzanol

[0117] The implementation steps of this embodiment are as follows:

[0118] A. Preparation of crude enzyme powder

[0119] Preparation of liquid enzyme production medium: Weigh 10 parts by weight of glucose, 0.3 parts by weight of NaCl, 1.3 parts by weight of ammonium sulfate, 0.3 parts by weight of magnesium sulfate heptahydrate, and 0.3 parts by weight of dipotassium hydrogen phosphate, and then add 0.5 parts by weight of saturated oryzanol two Methyl sulfoxide solution, and then add them to 1000 parts by weight of distilled water to dissolve, and use 0.3N aqueous sodium hydroxide solution or 0.2N nitric acid aqueous solution to adjust the pH of the obtained uniform solution to 7.0, and then use sterilizing equipment at a temperature of 121 ° C Sterilize under 20min, obtain described liquid enzyme production medium;

[0120] Pick a single colony of Burkholderia gladiolus A1-07 and inoculate it i...

Embodiment 2

[0127] Embodiment 2: the present invention prepares ferulic acid by oryzanol

[0128] The implementation steps of this embodiment are as follows:

[0129] A. Preparation of crude enzyme powder

[0130] Preparation of liquid enzyme production medium: Weigh 10 parts by weight of glucose, 0.3 parts by weight of NaCl, 1.3 parts by weight of ammonium sulfate, 0.3 parts by weight of magnesium sulfate heptahydrate, and 0.3 parts by weight of dipotassium hydrogen phosphate, and then add 0.5 parts by weight of saturated oryzanol two Methyl sulfoxide solution, and then add them to 1000 parts by weight of distilled water to dissolve, and use 0.4N potassium hydroxide aqueous solution or 0.3N hydrochloric acid aqueous solution to adjust its pH to 7.0, and then use sterilizing equipment at a temperature of 121 ° C Sterilize under 20min, obtain described liquid enzyme production medium;

[0131] Pick a single colony of Burkholderia gladiolus A1-07 and inoculate it into the liquid enzyme-pr...

Embodiment 3

[0138] Embodiment 3: the present invention prepares ferulic acid by oryzanol

[0139] The implementation steps of this embodiment are as follows:

[0140] A. Preparation of crude enzyme powder

[0141] Preparation of liquid enzyme production medium: Weigh 10 parts by weight of glucose, 0.3 parts by weight of NaCl, 1.3 parts by weight of ammonium sulfate, 0.3 parts by weight of magnesium sulfate heptahydrate, and 0.3 parts by weight of dipotassium hydrogen phosphate, and then add 0.5 parts by weight of saturated oryzanol two Methyl sulfoxide solution, and then add them to 1000 parts by weight of distilled water to dissolve, and use 0.1N sodium carbonate aqueous solution or 0.1N sulfuric acid aqueous solution to adjust the pH of the obtained uniform solution to 7.0, and then use sterilizing equipment at a temperature of 121 ° C Sterilize for 20 minutes to obtain the liquid enzyme-producing medium;

[0142] Pick a single colony of Burkholderia gladiolus A1-07 and inoculate it i...

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Abstract

The invention relates to burkholderia gladioli A1-07 and application of the burkholderia gladioli A1-07 in preparation of ferulic acid. The preparation method of the ferulic acid comprises the steps of preparing crude enzyme powder, hydrolyzing oryzanol and separating. The oryzanol conversion rate of the ferulic acid preparation method reaches 67%, the yield of the ferulic acid preparation method can reach more than 56%, and the purity of the obtained ferulic acid product is 22-31% by weight. Therefore, the ferulic acid preparation method disclosed by the invention is simple and convenient to operate, high in production efficiency and environment-friendly, and has a good industrial application prospect.

Description

【Technical field】 [0001] The invention belongs to the technical field of microorganisms. More specifically, the present invention relates to Burkholderia gladioli A1-07 and its use in the preparation of ferulic acid. 【Background technique】 [0002] Ferulic acid, whose chemical name is 4-hydroxy-3-methoxycinnamic acid, is a derivative of cinnamic acid, which widely exists in the plant cell wall skeleton, and mostly exists as an ester structure in nature. Ferulic acid has good pharmacological and biological activities, so it is of high value in medicine, health care products, and cosmetic raw materials. [0003] Oryzanol is a mixture of cycloartenyl ferulic acid esters and sterol ferulic acid esters. It is very rich in the by-products of rice bran oil refining. It has been produced by mature technology and has been industrialized in China. [0004] There are currently several methods of preparing ferulic acid. The hydrolysis method is to hydrolyze the angelica, wheat bran, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/42C12R1/01
CPCC12P7/42C12N1/20
Inventor 孟永宏胡永员郭建琦牛永洁杨璐
Owner SHAANXI HEALTHFUL BIOLOGICAL ENG
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