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Vibration device for immunofluorescence method

A vibrating device and immunofluorescence technology, which is applied in the direction of vibrating fluid, material inspection products, biological testing, etc., can solve problems such as cross contamination, low work efficiency, and long detection time, and achieve the effect of improving experimental efficiency

Pending Publication Date: 2021-11-23
SHAANXI MYBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a vibrating device for immunofluorescence to solve the problems of long detection time, low work efficiency and easy cross-contamination in the prior art

Method used

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  • Vibration device for immunofluorescence method
  • Vibration device for immunofluorescence method
  • Vibration device for immunofluorescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Apply the above-mentioned vibrating device for immunofluorescence to cell-based immunofluorescence, the cell slide is a cell slide overexpressing Hu antigen, the primary antibody is a positive sample against Hu antigen, specifically including The following steps:

[0043] (1) wash the slide with PBS;

[0044] (2) Shaking incubation with primary antibody: incubate positive samples for 15 minutes with primary antibody;

[0045] (3) Vibration washing: use PBST to wash 3 times;

[0046] (4) Secondary antibody vibration incubation: secondary antibody incubation time 15min;

[0047] (5) Vibration washing: wash 3 times with PBST;

[0048] (6) Observe the results under a microscope.

[0049] Apply the vibration device above for immunofluorescence to tissue-based immunofluorescence, the tissue slide is mouse hypothalamus, and the primary antibody is a positive sample of anti-AQP4 antigen, specifically including the following steps:

[0050] (1) Shaking incubation ...

Embodiment 2

[0070] Embodiment 2, adopt figure 1 The vibration device used for immunofluorescence method, the motor is a linear motor, wherein 60 motors 4 with an amplitude of 205 Hz are distributed on the motor base 5, the length, width and height of the motor 4 are 12 mm, 4 mm and 3 mm respectively, and the rated input voltage is 2.0±0.05Vrms AC, the connection mode of each motor 4 is parallel connection, and the cooling device 3 adopts a semiconductor refrigeration chip; the vibration device used for immunofluorescence method is applied to the cell-based immunofluorescence method, and the cell slide is the Cell slides expressing Tr antigen, the primary antibody is a positive sample against Tr antigen, specifically including the following steps:

[0071] (1) wash the slide with PBS;

[0072] (2) Shaking incubation with primary antibody: incubate positive samples for 15 minutes with primary antibody;

[0073] (3) Vibration washing: use PBST to wash 3 times;

[0074] (4) Secondary antib...

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Abstract

The invention discloses a vibration device for an immunofluorescence method, and belongs to the field of medical detection devices. The vibration device for the immunofluorescence method comprises a cavity, a vibration unit, a cooling device and sound insulation cotton. The sound insulation cotton is adopted to reduce noise in the experiment process, and the cooling device reduces heat generated by vibration, so that the heat generated by vibration is prevented from affecting a sample; and after being electrified, the vibration unit forms a vibration source, and a glass slide in an incubation box in the cavity is driven to vibrate through vibration, so that the exposure of antigen / antibody epitopes fixed onto the glass slide is promoted, the combination with antibodies / antigens in an incubation sample is accelerated, the incubation time is shortened, and the experiment efficiency is improved.

Description

technical field [0001] The invention belongs to the field of medical detection devices and relates to a vibration device for immunofluorescence. Background technique [0002] Immunofluorescence is a technology for antigen localization by labeling antibodies with fluorescent substances. This method is characterized by strong specificity, and the signal intensity contrast between positive and negative samples is obvious. Microscopic observation can accurately determine the distribution of fluorescence in tissues or cells. Now Has become a standard technique for autoantibody diagnosis. Although the immunofluorescence method has been widely used in the detection process of autoantibodies, the incubation time of the primary antibody and the secondary antibody for the existing immunofluorescence method is 30min-1h or even longer, and the whole detection process is time-consuming and difficult to detect. low efficiency. [0003] At present, it has been reported in the literature ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B06B1/10B06B3/00G01N33/53
CPCB06B1/10B06B3/00G01N33/53
Inventor 闫亚平程静美封雪李科
Owner SHAANXI MYBIOTECH CO LTD
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