Recombinant yarrowia lipolytica as well as construction method and application thereof

A technology of Yarrowia lipolytica and Yarrowia lipolytica, applied in the field of bioengineering, can solve problems such as poor heterologous high-efficiency expression effect, and achieve the effects of enhancing homologous recombination ability, improving genetic stability, and efficient construction method
CN113717871APending Publication Date: 2021-11-30NANJING NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING NORMAL UNIVERSITY
Publication Date
2021-11-30

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Abstract

The invention discloses recombinant yarrowia lipolytica as well as a construction method and application thereof. The recombinant yarrowia lipolytica is obtained by inserting a codon-optimized cyclooxygenase OAC gene expression cassette and a polyketide synthase OLS gene expression cassette into a yarrowia lipolytica genome. The recombinant yarrowia lipolytica disclosed by the invention is used for efficiently synthesizing olivetolic acid, the construction method is efficient, the operation is simple, and the olivetolic acid can be efficiently produced in a green manner.
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Description

technical field

[0001] The invention belongs to the technical field of bioengineering and relates to a recombinant Yarrowia lipolytica, in particular to a recombinant Yarrowia lipolytica and its construction method and application. Background technique

[0002] In addition to its antimicrobial, cytotoxic and photoprotective activities, the monoaromatic compound olivolic acid (OA) can also act as an alkyl resorcinol during the biosynthesis of cannabinoids. It has broad application prospects in the synthesis of pharmacological compounds.

[0003] At present, the acquisition of OA and its derivatives still relies on direct extraction from plants. This plant extraction method is severely limited by plant growth cycles and conditions. Geographical and climatic conditions significantly affect the quality of the synthesized OA precursor compounds. The extraction cycle extremely long. However, the existing chemical synthesis methods of OA often have complicated steps and low yield...

Claims

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