Recombinant Yarrowia lipolytica producing (-)-α-bisabolol and its construction method and application

A technology for lipolysis of Yarrowia and bisabolol, applied in the field of bioengineering, can solve the problems of the reduction of the number of eucalyptus brasiliensis, the complicated chemical synthesis steps, the difficulty of subsequent chiral separation, etc., so as to enhance the ability of homologous recombination and improve the genetic Stability, efficient construction methods
CN109913380BActive Publication Date: 2021-12-10NANJING TECH UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING TECH UNIV
Publication Date
2021-12-10

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Abstract

The invention provides a recombinant Yarrowia lipolytica for producing (−)-α-bisabolol, a construction method and application thereof, and relates to the field of bioengineering. The recombinant Yarrowia lipolytica that produces (−)‑α‑bisabolol is inserted into the Yarrowia lipolytica genome with (‑)‑α‑bisabolol synthase, farnesyl pyrolysis Phosphate synthase and 3‑hydroxy‑3‑methylglutaryl CoA reductase expression cassettes. The recombinant Yarrowia lipolytica of the present invention can efficiently synthesize (-)-α-bisabolol, the construction method is efficient, and the operation is simple.
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Description

technical field

[0001] The invention relates to the field of bioengineering, in particular to a recombinant Yarrowia lipolytica for producing (-)-α-bisabolol and its construction method and application. Background technique

[0002] (-)-α-Bisabolol is an unsaturated sesquiterpene alcohol mainly found in the essential oils of Brazilian eucalyptus (Eremanthuserythropappus) and German chamomile (Matricaria recutita). (-)-α-Bisabolol has been widely used in oral hygiene products and skin care products due to its good anti-inflammatory, antibacterial, antiseptic and moisturizing properties, and has been added to big brands such as Estee Lauder and Clinique in skin care products. The U.S. Food and Drug Administration (FDA) has approved (-)-α-bisabolol as a safe ingredient to be safely added to various daily chemical products such as mouthwash, skin care products, and shower gels (Waltimo T et al. al., Arch Oral Biol. 2013, 58:10-6).

[0003] At present, (-)-α-bisabolol is mainl...

Claims

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