Collagen proteolytic enzyme and its coding gene, preparation method and application

Abstract

The invention discloses a collagen proteolytic enzyme, the amino acid sequence of which is shown in SEQ ID NO.1. The invention also discloses the collagen proteolytic enzyme gene, the collagen proteolytic enzyme gene recombination carrier, the collagen proteolytic enzyme gene engineering bacteria, the preparation method of the collagen proteolytic enzyme and the application of the collagen proteolytic enzyme. The collagen proteolytic enzyme of the invention can degrade bone collagen and increase the yield of small molecular weight bone collagen peptide.

Description

technical field [0001] The present invention relates to the field of biotechnology. More specifically, the present invention relates to a collagen hydrolytic enzyme and its encoding gene, preparation method and application. Background technique [0002] Livestock and poultry bones are rich in collagen, which is an important raw material for preparing functional collagen peptides and has great utilization value. The small molecular weight collagen peptide prepared by enzymatic hydrolysis of livestock and poultry bone collagen has anti-oxidation, anti-aging, promotion of mineral absorption, anti-osteoporosis and other activities. At the same time, the small molecular weight collagen peptide has the characteristics of good solubility and easy absorption, and the absorption effect is better than that of collagen. Protease hydrolysis is currently the most commonly used method for preparing small molecular weight collagen peptides, which has the advantages of low cost, simple op...

AI Technical Summary

Problems solved by technology

However, due to the unique (G-X-Y)n repeating unit sequence and dense triple helix structure of collagen, only a very small number of proteases have the ability to catalyze the hydrolysis of bone collagen, and there are few commercially available collagenases and production strains

Research has shown that, Clostridium histolyticum , Bacillus sp., Candida albicans and Vibrio vulnificus etc. have the potential to produce collagen hydrolytic enzymes, but most of these strains are pathogenic bacteria. When producing collagen hydrolytic enzymes, the pathogenic bacteria also produce corresponding toxins, which are not suitable for the preparation of bone-derived foods.

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