Kit simultaneously used for aedes classification monitoring and dengue virus detection

Abstract

The invention provides a kit simultaneously used for aedes classification monitoring and dengue virus detection. The kit comprises the following reagents: an RT-qPCR premix solution, RT-qPCR enzyme, a positive quality control product and a negative quality control product. The RT-qPCR premixed solution is prepared from a one-step reaction RT-qPCR buffer solution, a forward specific primer of a highly conserved sequence in a non-coding region of dengue virus, a reverse specific primer of the highly conserved sequence in the non-coding region of the dengue virus and a dengue virus probe; an aedes aegypti ribosome RNA forward specific primer, an aedes aegypti ribosome RNA reverse specific primer and an aedes aegypti ribosome RNA probe, an aedes albopictus ribosome RNA forward specific primer, an aedes albopictus ribosome RNA reverse specific primer and an aedes albopictus ribosome RNA probe. The kit has the advantages of sensitivity, accuracy and high efficiency.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a kit for the classification monitoring of Aedes mosquitoes and the detection of dengue virus. [0002] technical background [0003] Dengue virus (dengue virus) is a single-stranded positive-sense RNA virus, which belongs to the Flavivirus genus of the family Flaviviridae. It is a group of viruses that are highly conserved at the level of virus structure and genome. Dengue virus is transmitted through mosquito vectors, the main vectors are Aedes aegypti and Aedes albopictus, causing dengue fever, dengue hemorrhagic fever and dengue shock syndrome with high morbidity and mortality. Morphologically, dengue virus is an enveloped spherical virus with a diameter of about 50nm. Since the dengue virus E protein forms dimers in an end-to-tail manner and regularly arranges on the surface of the virus, it presents a conformation of 20-hedron symmetry. The genome RNA of dengue virus contains abo...

AI Technical Summary

Problems solved by technology

[0008] The object of the present invention is to provide a kind of test kit that is used for Aedes mosquito classification monitoring and dengue virus detection at the same time, aiming to solve the long time for classification and identification of mosquito eggs, pupae and larvae in the prior art, mosquito complexes, close relatives, etc. When important signs such as species and adult mosquito abdominal scales or wings fall off and are damaged, it is impossible to classify and identify, and it is impossible to detect dengue virus while carrying out mosquito vector classification and identification.

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