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Calcium-containing micro-precipitation lipidosome entrapped with photothermal agent and chemotherapeutic drug as well as preparation method and application of calcium-containing micro-precipitation lipidosome

A technology for chemotherapeutic drugs and photothermal agents, which is applied in the directions of liposome delivery, drug combination, pharmaceutical formulations, etc., can solve the problems such as the difficulty of photothermal agents to play a therapeutic role, the poor selectivity of chemotherapeutic drugs, and the instability of photothermal conversion. , to improve the anti-tumor effect, improve the therapeutic effect, and achieve the effect of less side effects

Pending Publication Date: 2021-12-07
新疆生产建设兵团医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, photothermal agents are easy to diffuse in the body and repeated irradiation leads to unstable photothermal conversion, making it difficult for photothermal agents to exert their therapeutic effects; chemotherapy drugs are not selective, and they will inevitably damage cancer cells while killing cancer cells. Normal cells of the human body, resulting in adverse drug reactions

Method used

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  • Calcium-containing micro-precipitation lipidosome entrapped with photothermal agent and chemotherapeutic drug as well as preparation method and application of calcium-containing micro-precipitation lipidosome
  • Calcium-containing micro-precipitation lipidosome entrapped with photothermal agent and chemotherapeutic drug as well as preparation method and application of calcium-containing micro-precipitation lipidosome
  • Calcium-containing micro-precipitation lipidosome entrapped with photothermal agent and chemotherapeutic drug as well as preparation method and application of calcium-containing micro-precipitation lipidosome

Examples

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preparation example Construction

[0041] The present invention provides a method for preparing calcium-containing microprecipitated liposomes containing photothermal agents and chemotherapeutic drugs described in the above technical scheme, comprising the following steps:

[0042] (1) Mixing water-soluble calcium salts, chemotherapeutic drugs and water to obtain an aqueous phase of chemotherapeutic drugs; adding the aqueous phase of chemotherapeutic drugs to the oil phase for ultrasonic dispersion to obtain a mixed and dispersed oil phase of chemotherapeutic drugs; the oil phase includes Ionic surfactants and water-immiscible organic solvents;

[0043] (2) Mix the acid radical-containing anion compound, bovine serum albumin, photothermal agent and water to obtain the photothermal agent water phase; add the photothermal agent water phase to the oil phase for ultrasonic dispersion, add the inner layer phospholipid material and mix , to obtain a photothermal agent mixed and dispersed oil phase; the oil phase incl...

Embodiment 1

[0061] (1) Calcium chloride and doxorubicin (DOX) were dissolved in 5 mL deionized water to obtain a mixed solution of chemotherapeutic drugs, and 300 μL of the mixed solution of chemotherapeutic drugs was added dropwise to the In the mixed oil phase of cyclohexane / nonoxynol ether of 15mL, continue to sonicate until the water droplets are cracked and evenly dispersed in the mixed oil phase to obtain the mixed dispersed oil phase of chemotherapy drugs; the calcium chloride in the mixed solution of chemotherapy drugs The concentration is 500mmol / L, and the concentration of DOX is 1mg / mL; the volume ratio of cyclohexane and nonoxynol ether in the mixed oil phase is 70:30;

[0062] (2) Sodium hydrogen phosphate, bovine serum albumin (BSA) and indocyanine green (ICG) were dissolved in 5 mL deionized water to obtain a photothermal agent mixed solution; Add the photothermal agent mixed solution dropwise into 15mL of cyclohexane / nonoxynol mixed oil phase, continue to sonicate until th...

Embodiment 2

[0066] According to the method of Example 1, the calcium-containing liposomes loaded with photothermal agents and chemotherapeutic drugs are prepared, and the difference from Example 1 is that the sodium hydrogen phosphate in step (2) is replaced by sodium phosphate; step (3) obtains BSA / photothermal agent / chemotherapy drug / calcium phosphate microprecipitation.

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Abstract

The invention provides a calcium-containing micro-precipitation liposome entrapped with a photothermal agent and a chemotherapeutic drug as well as a preparation method and application of the calcium-containing micro-precipitation liposome, and belongs to the technical field of liposomes. The calcium-containing micro-precipitation liposome entrapped with the photothermal agent and the chemotherapeutic drug comprises a calcium-containing micro-precipitation inner core and a phospholipid bilayer shell entrapped outside the calcium-containing micro-precipitation inner core, the calcium-containing micro-precipitation core comprises the photothermal agent, the chemotherapeutic drug, bovine serum albumin and a calcium-containing precipitate; the phospholipid bilayer shell comprises an inner-layer phospholipid material, an outer-layer phospholipid material and cholesterol. According to the calcium-containing micro-precipitation liposome entrapped with the photothermal agent and the chemotherapeutic drug, the half-life period of the photothermal agent is prolonged, the stability is improved, and the treatment effect of the photothermal agent on tumor cells is improved; the toxic and side effects of chemotherapeutic drugs can be obviously reduced, and the therapeutic effect on the tumor cells is improved; the photothermal-chemical combined treatment can be realized, the side effect is small, and the positioning and treatment effects on the tumor cells are excellent.

Description

technical field [0001] The invention relates to the technical field of liposomes, in particular to calcium-containing microprecipitated liposomes loaded with photothermal agents and chemotherapeutic drugs, a preparation method and application thereof. Background technique [0002] As one of the standard treatments for cancer, chemotherapy suffers from a dilemma due to lack of tissue targeting and dose-limiting toxicity. With the rapid development of nanomedicine, scientists have developed many new anticancer therapeutic strategies, such as photodynamic therapy (PDT), radiation therapy, gene therapy, immunotherapy, photothermal therapy (PTT), and chemotherapy. But single therapy is no longer enough to keep up with the progress of cancer, and many combination therapies have been used clinically, among which, the combination of chemotherapy and photothermal therapy has become a promising strategy. [0003] Photothermal therapy (PTT), which uses near-infrared (NIR) light to irr...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K41/00A61K9/127A61K47/42A61K47/24A61K47/28A61K47/02A61K47/12A61P35/00
CPCA61K45/06A61K41/0052A61K9/127A61K47/42A61K47/24A61K47/28A61K47/02A61K47/12A61P35/00A61K2300/00
Inventor 周旭坤刘卫庄峰刘海滨
Owner 新疆生产建设兵团医院
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