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Hybrid fiber sponge with hemostasis and wound repair functions and preparation method thereof

A hybrid fiber and wound repair technology, which is applied in the field of hybrid fiber sponge and its preparation, can solve the problems of unstable curative effect, weak hemostatic effect, limited indication range, etc., achieve excellent mechanical properties and shape recovery, improve the The effect of poor bacterial resistance

Active Publication Date: 2021-12-10
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a single chitosan material has the disadvantages of weak hemostatic effect and unsatisfactory antibacterial ability, its curative effect is unstable, and the range of indications is limited.

Method used

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  • Hybrid fiber sponge with hemostasis and wound repair functions and preparation method thereof
  • Hybrid fiber sponge with hemostasis and wound repair functions and preparation method thereof
  • Hybrid fiber sponge with hemostasis and wound repair functions and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] In the present embodiment, prepare gallic acid modified chitosan (CS-GA), the steps are as follows:

[0059] (1) Dissolve chitosan (CS) with a molecular weight of about 200kDa in aqueous hydrochloric acid solution, add 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) , N-hydroxysuccinimide (NHS) and gallic acid (GA), use 0.1mol / L and 1mol / L NaOH solution, and 0.1mol / L and 1mol / L HCl solution to adjust and maintain the pH of the reaction solution The value is between 4.75 and 5, and the reaction is performed under nitrogen protection for 15 to 24 hours; in this step, the mass of CS, NHS, EDC·HCl and GA is 129:102:170:152.

[0060] (2) Transfer the reaction solution obtained in step (1) into a dialysis bag with cut off MW=8000-13000, dialyze in a hydrochloric acid solution with a pH value of 3.3-3.5 for 5 days, and freeze-dry to obtain gallic acid modified shells Glycans (CS-GA).

[0061] The proton nuclear magnetic resonance spectra of the CS-GA pre...

Embodiment 2

[0065] In the present embodiment, silk fibroin (SF) is extracted from silkworm cocoons, and the steps are as follows:

[0066] (1) Cut silkworm cocoons into pieces and peel them off, put them into boiling 0.5% sodium bicarbonate aqueous solution for 30 minutes according to the bath ratio of 1:100, repeat the operation twice, then rinse with deionized water, and air-dry in a fume hood.

[0067] (2) Add the dried desericin silk obtained in step (1) into 9.5mol / L lithium bromide solution, configure it into a 10% (w / v) solution, stir at room temperature for 3 to 4 days, and use the gained reaction solution with Diluted with distilled water, transferred to a dialysis bag with cut off MW=8000-13000, dialyzed with distilled water for 3 days, and freeze-dried.

[0068] (3) Place (2) freeze-dried product obtained in calcium chloride-water-ethanol mixed solution (the molar ratio of calcium chloride, water and ethanol is 1:8:2) with a bath ratio of 1:10. After stirring at 73°C for 1 h, ...

Embodiment 3

[0071] In this example, the preparation of a hybrid fiber sponge with both hemostasis and wound repair, the steps are as follows:

[0072] (1) Dissolve the CS-GA prepared in Example 1 and the SF prepared in Example 2 in deionized water to form a mixed solution. In this mixed solution, the mass ratio of CS-GA to SF is 1:1, and CS-GA and The concentration of SF is all 3wt.%. Then according to the mass ratio of sodium periodate and CS-GA is the ratio of 1:10, in the mixed solution, adding concentration is the sodium periodate solution of 5mg / mL, shakes and mixes immediately and obtains reaction solution, centrifuge The air bubbles in the reaction solution are removed by centrifugation, poured into a mold, and left to stand at room temperature until each component is fully cross-linked to obtain a hydrogel with a composite three-dimensional network structure.

[0073] (2) Freeze-dry the hydrogel with a composite three-dimensional network structure obtained in step (1) to obtain a...

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Abstract

The invention provides a hybrid fiber sponge with hemostasis and wound repair functions and a preparation method thereof. The hybrid fiber sponge is formed by processing after soaking a freeze-dried sponge in an ethanol solution of 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, wherein the freeze-dried sponge is formed by freeze-drying hydrogel formed by crosslinking polyphenol modified chitosan and a protein material and has a composite three-dimensional network structure; and in the process that the freeze-dried sponge is soaked in the ethanol solution of 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, carboxyl and amino in the freeze-dried sponge form amido bonds, and meanwhile, the protein material in the freeze-dried sponge are denatured to form beta folds, so that the hybrid fiber sponge is of a porous structure. The hybrid fiber sponge provided by the invention can take effects of inhibiting bacteria and promoting tissue regeneration while stopping bleeding, and is suitable for various bleeding models such as body surface oppression bleeding and in-vivo non-oppression bleeding.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a hybrid fiber sponge capable of hemostasis and wound repair and a preparation method thereof. The hybrid fiber sponge has good application prospects in hemostasis, wound repair, tissue engineering and the like. Background technique [0002] According to reports, more than 30% of traumatic deaths worldwide are caused by excessive blood loss. At the same time, bleeding is often accompanied by the risk of bacterial infection and skin loss, which seriously threatens the life and health of patients. Therefore, the development of hemostatic materials with rapid hemostatic effect, which can inhibit bacterial infection and promote wound healing is of vital significance for clinical treatment. Although existing hemostatic materials such as gelatin, chitosan, glutaraldehyde cross-linked albumin, and fibrin-based bandages have good hemostatic efficiency for compressible bleeding on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00A61L24/08A61L26/00C08B37/08
CPCA61L24/0036A61L24/0042A61L24/08A61L24/10A61L26/0085A61L26/009A61L26/0023A61L26/0028C08B37/003A61L2400/04C08L5/08
Inventor 孙勇樊渝江王玉祥
Owner SICHUAN UNIV
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