Preparation method of low-mineralization micelle-state casein

A casein and casein glue technology, applied in animal protein processing, protein food ingredients, milk protein ingredients, etc., can solve the problems of difficult to accurately control the decalcification rate, exploration of conditions and cumbersome overall treatment process, etc. The effect of small binding state and protein properties, high practical application value, and simple process

Pending Publication Date: 2021-12-21
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] When the existing process prepares batches of ingredients with different decalcification rates, it is necessary to repeat the decalcification operation steps continuously, and the decalcification rate is difficult to control accurately, and the pre-condition exploration and overall treatment process are relatively cumbersome

Method used

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  • Preparation method of low-mineralization micelle-state casein
  • Preparation method of low-mineralization micelle-state casein
  • Preparation method of low-mineralization micelle-state casein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation process of non-decalcified micellar casein

[0039] Take fresh milk for disc centrifugal degreasing treatment (fat content is 0.09%), and then use 1.4μm pore size ceramic membrane for sterilization treatment to obtain sterilized skim milk, and then use 100nm microfiltration ceramic membrane to separate casein micelles from the sample , the concentration factor is 5 times, and then supplemented with deionized water for 3 times of diafiltration to reduce the residue of impurities such as whey protein and improve the purity of casein. micelles.

Embodiment 2

[0040] Embodiment 2 high decalcification casein preparation technology

[0041] The main process such as figure 1 -As shown in -A, at first adopt 100nm microfiltration ceramic membrane to separate the casein micelles in the sterilized skim milk (same as Example 1), the concentration factor adopted is 5 times, then add deionized water and carry out diafiltration for 3 times, the temperature Control at 60°C to obtain undecalcified casein micelles. Then the acidification method or ion exchange method can be used to prepare high calcium-depleted casein, and the specific steps are as follows:

[0042] 1. Acidification method: Add 4 times the volume of deionized water to the separated non-decalcified casein micelles, and then add 35mM glucosinolate-δ-lactone to adjust the pH to 5.3. Then, an ultrafiltration membrane with a pore size of 10 kDa was used for ultrafiltration and diafiltration, the concentration factor was 5 times, the number of diafiltration was 3 times, and the treat...

Embodiment 3

[0044] Embodiment 3 one-step low mineralization micellar state casein preparation technology

[0045] The traditional one-step process for preparing low-mineralized casein is as follows: figure 1 As shown in -B, the non-decalcified casein micelles obtained in Example 1 were supplemented with 4 times the volume of deionized water, and then 12 mM glucosinolate-δ-lactone was added to adjust the pH value to 6.2. Afterwards, an ultrafiltration membrane with a pore size of 10 kDa was used for ultrafiltration and diafiltration. The concentration factor was 5 times, and the number of diafiltration was 3 times. The treatment temperature was controlled at 60 ° C, and then the pH was adjusted back to the initial value. Spray drying was carried out after the condition of 25°C was maintained for 60 minutes, and the inlet temperature and outlet temperature were 190°C and 90°C, respectively. Known through measuring, the decalcification rate at this moment is 28%.

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Abstract

The invention discloses a preparation method of low-mineralization micelle-state casein, and belongs to the field of milk powder processing. The method comprises the following steps: firstly, carrying out microfiltration and multi-time washing filtration on sterilized skimmed milk to obtain a casein micelle ingredient, then adjusting a part of the ingredient to a relatively low pH condition by using an acidifying agent to dissociate micelle calcium phosphate, and removing free calcium by combining ultrafiltration and multi-time washing filtration, or directly removing free calcium and micelle calcium in the ingredient by adopting a cation exchange column; and after the pH value is adjusted back to the initial pH value, compounding the decalcified casein ingredient with non-decalcified casein to realize preparation at different mineralization levels, and then performing spray drying to obtain a series of low-mineralization casein ingredients. The method is simple and convenient in process, the preparation of a series of casein ingredients with different decalcification rates can be quickly realized to meet different use requirements, the control on the decalcification rate is accurate, and the standardization of the product quality is realized.

Description

technical field [0001] The invention relates to a method for preparing low mineralized micellar casein, which belongs to the field of milk powder processing. Background technique [0002] Casein is an important nutritional component in milk, and its content accounts for about 80% of the total protein. As a full-valent protein, casein contains 8 kinds of amino acids necessary for the human body, and is also an important source of calcium and phosphorus for the body. In addition, it has good emulsifying, foaming and gelling properties, making casein Protein products are widely used in the food industry. In order to maintain the bioactive components in whey protein, membrane filtration is used to directly separate from skim milk, which has become a research and development hotspot of whey protein at present. This preparation method will produce micellar casein by-products. Specific developments help to increase the overall utilization value of dairy raw materials. The molecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/20A23J3/10A23C9/152
CPCA23J1/202A23J3/10A23C9/1526
Inventor 周鹏刘大松王科瑜
Owner JIANGNAN UNIV
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