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Potted plant detection device and detection method for effects of microbial volatile substances on plants

A technology of volatile substances and detection devices, which is applied in the field of research on the effect of microorganisms on plant growth, can solve the problem of limited space in two-compartment petri dishes, difficulty in distinguishing the action sites of volatile metabolites, and inability to apply to plants with large growth sizes, etc. problems, to achieve the effect of convenient inoculation of strains and control of the internal environment

Active Publication Date: 2021-12-21
GUANGXI UNIV FOR NATITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, to study the effect of microbial volatile metabolites on plant growth, the culture method of two-compartment petri dish is usually used for plate experiments. The disadvantage of this culture method is that the space of two-compartment petri dishes is limited, and only the effect of microbial volatile metabolites on plant growth can be studied. effect, and cannot be applied to faster growing, larger plants
At the same time, the stem, leaf and root system are in an environment, it is difficult to distinguish the action sites of volatile metabolites, and the volatile metabolites are not easy to act on the root system through the medium
[0004] In the process of co-cultivation of plants and microorganisms, there are problems such as that it is difficult to co-cultivate microorganisms and plants, it is difficult for volatile metabolites of microorganisms to directly act on plant roots, and it is difficult to directly observe the interaction between microorganisms and plants.
At present, there is no fixed experimental device in the field of studying the effect of microbial volatile metabolites on plants, and a pot experiment detection device is needed for practical application

Method used

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  • Potted plant detection device and detection method for effects of microbial volatile substances on plants
  • Potted plant detection device and detection method for effects of microbial volatile substances on plants
  • Potted plant detection device and detection method for effects of microbial volatile substances on plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The integrated container and the bottom tank are all made of glass or ceramics. The detection method is as follows:

[0070] Step1: The bottom tank mouth 9 is airtightly connected with the connection structure 6, and the bottom tank is connected and combined with the integrated container; the inner cavity 11 of the bottom tank forms a cavity, and the opening of the plant cultivation area 7 is upward, and the sealing cover or film is added to seal the plant cultivation area 7 After the opening, the inner cavity 11 of the bottom tank forms a sealed internal environment, and forms a sterile internal environment after sterilization;

[0071] Step2: Put the integrated container upside down on the ultra-clean workbench after ultraviolet sterilization, open the bottom tank, then pour the high-temperature sterilized solid medium into the microbial culture area 2, and then connect the connection structure through the bottom tank port 9 6 Sealed connection, bottom tank and integr...

Embodiment 2

[0083] The overall material of the device is plastic. For recyclable plastic materials, the present invention can be sterilized and recycled according to Embodiment 1. The feature of this embodiment is that the device can be designed and produced as a disposable experimental device due to its simple design, and the disposable experimental device can be designed and molded by injection molding, the production process is simple and efficient, and it can be mass-produced. The product is aseptically processed and packaged. There is no need for secondary sterilization when using it. It can be opened and used directly in the ultra-clean bench. It can be taken as needed, which helps to improve work efficiency and avoid secondary pollution.

Embodiment 3

[0085] Compared with the first embodiment, this embodiment is different in usage. This embodiment adopts the experimental method of pre-filling the culture soil and then sterilizing the closed container as a whole. The specific steps are:

[0086] Step1: After filling the plant cultivation area 7 with cultivation soil, add a sealing cover or a film to seal it, connect the connection structure 6 through the bottom tank mouth 9, and connect and combine the bottom tank with the integrated container, and the inner cavity 11 of the bottom tank forms a sealed Internal environment;

[0087] Step2: Put the integrated container into the sterilization pot for overall sterilization, and the inner cavity 11 of the bottom tank will form a sterile environment after sterilization;

[0088]Step3: Put the integrated container upside down on the ultra-clean workbench, pour the high-temperature sterilized solid medium into the microbial culture area 2, after the solid medium is cooled, open th...

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Abstract

The invention discloses a potted plant detection device and a detection method for effects of microbial volatile substances on plants, and solves the problems of ensuring pure culture of microorganisms and facilitating observation of effects of volatile metabolites of the microorganisms on the plants through combined integrated design of a plant cultivation area and a microorganism culture area. A porous structure of the plant cultivation area enables volatile metabolites to freely pass through and contact root systems, and the water storage area keeps soil humidity. The microorganism culture area is blocked by an annular partition plate to form an annular area surrounding the plant culture area, and it is guaranteed that volatile metabolites directly act on plant roots. A connecting structure enables a plant cultivation and microorganism culture integrated container to be tightly connected with the bottom tank, the inner cavity of the bottom tank is in a sterile environment after sterilization, and infectious microbe pollution is prevented. The device and the method are suitable for research on potted plant experiment detection in the aspect of effects of the microbial volatile metabolites on plants.

Description

technical field [0001] The invention relates to the technical field of research on the effect of microorganisms on plant growth, in particular to a potted detection device and method for detecting the effect of microbial volatile substances on plants. Background technique [0002] Plants will be affected by microorganisms during their growth in the soil. Beneficial microorganisms produce hormones, promote plant nutrient absorption, regulate plant metabolism, induce and activate resistance genes, modify and regulate key gene expressions, etc., or directly inhibit The indirect mode of action such as the growth of pathogenic bacteria promotes plant growth and development. In the study of tobacco, the volatile metabolite 1,3-butanediol produced by the symbiotic Enterobacter cloacae can significantly promote the growth of Arabidopsis plants, and can regulate the root architecture, the number of lateral roots and the length of the main root . Studies have shown that microbial vo...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12M1/00C12M1/12
CPCC12Q1/02C12M23/34C12M37/04Y02A50/20
Inventor 姜明国王锟丰景王金子高亚慧
Owner GUANGXI UNIV FOR NATITIES