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Centralized cell sorting system and sorting method

A sorting system and cell technology, applied in the field of cell sorting, can solve the problems of increased analysis time and cost, increased volume of recovered liquid, loss of precious samples, etc., so as to reduce analysis costs, improve efficiency, and avoid cell loss and damage. Effect

Pending Publication Date: 2021-12-28
SHENZHEN GENFLOW TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, it is generally necessary to dilute the sample before sorting or introduce buffer solution during sorting, which will lead to a significant increase in the volume of the recovered solution
When the recovered target cells are less than 1,000, the recovered samples need to be concentrated during subsequent analysis and identification, which can easily cause loss of precious samples during operation
In some solutions, the integration of sorting enrichment and on-chip filtration and capture is used to immobilize the enriched cells on a specific area of ​​the chip for on-chip analysis. However, in order to reduce the capture flow rate and improve the success rate of capture, the capture area The area is usually relatively large, so it is also faced with the problem that full-coverage scanning is required during analysis, which leads to increased analysis time and cost

Method used

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  • Centralized cell sorting system and sorting method
  • Centralized cell sorting system and sorting method
  • Centralized cell sorting system and sorting method

Examples

Experimental program
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Effect test

Embodiment 1

[0078] This embodiment provides a centralized cell sorting system, such as Figure 1-Figure 4 As shown, it includes a microfluidic cell sorting device 120 for enriching target cells 250 in a cell suspension sample containing target cells 250, and a first driver for driving the cell suspension sample into the microfluidic cell sorting device 120 mechanism; the microfluidic cell sorting device 120 is provided with a pipeline A150 for the sample waste liquid 230 to flow out and a pipeline B160 for the target cell 250 to flow out; Pore ​​filter device 110.

[0079] Specifically, the first driving mechanism may be a peristaltic pump, a syringe pump, a diaphragm pump, a gear pump, a plunger pump, an electroosmotic pump, etc., which are used to drive the cell suspension into the microfluidic cell sorting device 120 .

[0080] The microfluidic cell sorting device removes most of the non-target cells and other particulate components in the cell suspension sample, reducing and avoiding...

Embodiment 2

[0109] This embodiment provides a centralized cell sorting system, such as Figure 4 as shown, Figure 4 for figure 2A more specific implementation of the system shown is used to illustrate another possible presentation of the system when different types of component functional units are used. Including the pretreatment system 100 and the second device 360, wherein the microfluidic cell sorting device 120 can further be a spiral flow channel inertial focus microfluidic chip; the spiral flow channel inertial focus microfluidic chip includes a plurality of spirally arranged flow One end of the flow channel includes an inlet C for the input of the diluted cell suspension sample, and the other end includes an outlet D; the outlet D includes at least two channels, one of which is for target cells and buffer to flow out, and the other for samples Waste liquid outflow; in a specific application, the flow ratio of the sample waste liquid after flowing out of the spiral flow channel...

Embodiment 3

[0126] The present invention also includes a cell sorting method, using the above-mentioned centralized cell sorting system; such as Figure 6 As shown, which includes the following steps:

[0127]Step 1: introduce the cell suspension sample containing target cells into the microfluidic cell sorting device through the sample injection pipeline, and remove the main non-target particle components through the microfluidic cell sorting device;

[0128] Step 2: introduce the sorted cell suspension sample in step 1 into the microporous membrane device along the fixed flow channel, and the microporous membrane device filters out some non-target particle components and waste liquid, and simultaneously The target cells are trapped in the microporous membrane device to complete the sorting;

[0129] Step 3: separating the microporous membrane device containing the target cells retained in step 2 from the fixed channel.

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Abstract

The invention relates to a centralized cell sorting system and sorting method. The system comprises a microfluidic cell sorting device for performing target cell enrichment on a cell suspension sample containing target cells, and a first driving mechanism for driving the cell suspension sample to enter the microfluidic cell sorting device; a pipeline A for sample waste liquid to flow out and a pipeline B for target cells to flow out are arranged on the microfluidic cell sorting device; and a microfiltration membrane device for filtering the target cells is also arranged on the pipeline B. Most of particle components such as cells in the sample are removed through the microfluidic cell sorting device, the possibility that a filter membrane is blocked when the sample flows through the microfiltration membrane device is reduced and avoided, the target cells are separated from the sample waste liquid under the action of the microfluidic cell sorting device, cell sorting is achieved, and the target cells are further filtered through the microfiltration membrane device, so that the target cells are centralized in the microfiltration membrane device, and the cell sorting efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of cell sorting, and more specifically, to a centralized cell sorting system and a sorting method. Background technique [0002] In cell-based liquid biopsies, abnormal cells such as circulating tumor cells can be enriched from blood by size and firmness of cells and cell clusters. In the sorting and enrichment methods based on physical characteristics, they can be roughly divided into two types: microfluidic chip-based sorting and enrichment and microporous membrane-based sorting and enrichment. [0003] Due to the huge number of cells in blood, it brings great pressure for sorting and enrichment. Among them, in the separation and enrichment technology based on microporous membranes, conventional nuclear pore membranes are difficult to support blood filtration of more than 5 mL due to their low porosity. In order to increase the porosity while ensuring the uniformity of the micropore diameter, we can only...

Claims

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Application Information

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IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/12C12M1/04C12M1/00B01L3/00
CPCC12M23/16C12M29/00C12M29/04C12M33/04C12M33/14C12M37/04C12M41/12C12M41/40C12M41/46C12M47/04B01L3/502761
Inventor 关国峰钞书哲
Owner SHENZHEN GENFLOW TECH CO LTD
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