Centralized cell sorting system and sorting method

Abstract

The invention relates to a centralized cell sorting system and sorting method. The system comprises a microfluidic cell sorting device for performing target cell enrichment on a cell suspension sample containing target cells, and a first driving mechanism for driving the cell suspension sample to enter the microfluidic cell sorting device; a pipeline A for sample waste liquid to flow out and a pipeline B for target cells to flow out are arranged on the microfluidic cell sorting device; and a microfiltration membrane device for filtering the target cells is also arranged on the pipeline B. Most of particle components such as cells in the sample are removed through the microfluidic cell sorting device, the possibility that a filter membrane is blocked when the sample flows through the microfiltration membrane device is reduced and avoided, the target cells are separated from the sample waste liquid under the action of the microfluidic cell sorting device, cell sorting is achieved, and the target cells are further filtered through the microfiltration membrane device, so that the target cells are centralized in the microfiltration membrane device, and the cell sorting efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of cell sorting, and more specifically, to a centralized cell sorting system and a sorting method. Background technique [0002] In cell-based liquid biopsies, abnormal cells such as circulating tumor cells can be enriched from blood by size and firmness of cells and cell clusters. In the sorting and enrichment methods based on physical characteristics, they can be roughly divided into two types: microfluidic chip-based sorting and enrichment and microporous membrane-based sorting and enrichment. [0003] Due to the huge number of cells in blood, it brings great pressure for sorting and enrichment. Among them, in the separation and enrichment technology based on microporous membranes, conventional nuclear pore membranes are difficult to support blood filtration of more than 5 mL due to their low porosity. In order to increase the porosity while ensuring the uniformity of the micropore diameter, we can only...

AI Technical Summary

Problems solved by technology

For example, it is generally necessary to dilute the sample before sorting or introduce buffer solution during sorting, which will lead to a significant increase in the volume of the recovered solution

When the recovered target cells are less than 1,000, the recovered samples need to be concentrated during subsequent analysis and identification, which can easily cause loss of precious samples during operation

In some solutions, the integration of sorting enrichment and on-chip filtration and capture is used to immobilize the enriched cells on a specific area of ​​the chip for on-chip analysis. However, in order to reduce the capture flow rate and improve the success rate of capture, the capture area The area is usually relatively large, so it is also faced with the problem that full-coverage scanning is required during analysis, which leads to increased analysis time and cost

Images