Preparation method and application of shark chondroitin sulfate chelated zinc salt

A technology of chondroitin sulfate and shark cartilage, which is applied in the fields of application, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of no patent, no chondroitin sulfate chelated zinc salt, etc., and achieve bioavailability High, non-toxic and side effects, mild production conditions

Pending Publication Date: 2022-01-04
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no products of chondroitin sulfate chelated zinc salt are currently on the market, nor are there related patents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of shark chondroitin sulfate chelated zinc salt, comprising the following steps:

[0032] 1. Shark cartilage removes fat, shark meat, and calcified bones, and grinds it with a pulverizer to obtain 1kg of shark cartilage powder.

[0033] 2. Put the above-mentioned shark cartilage powder in a reaction kettle, add 10kg of water, add 5g of animal protease, use sodium hydroxide and sulfuric acid to adjust the pH value, and control the pH value of the reaction system to 6.5, and the enzymatic hydrolysis temperature is 65°C. The solution time is 6h. After the enzymatic hydrolysis reaction, use a centrifuge with a rotating speed of 8000rpm to remove solid impurities, and use a nanofiltration membrane with a molecular weight cut-off of 5kD to remove impurity proteins from the obtained clear liquid to obtain a shark chondroitin sulfate solution.

[0034] 3. Put the above-mentioned shark chondroitin sulfate solution in an ultrasonic reaction tank, add zinc su...

Embodiment 2

[0039] A preparation method of shark chondroitin sulfate chelated zinc salt, comprising the following steps:

[0040] 1. Shark cartilage removes fat, shark meat, and calcified bones. After pulverizing with a pulverizer, 5kg of shark cartilage powder is obtained.

[0041]2. Put the above-mentioned shark cartilage powder in a reaction kettle, add 50kg of water, add 50g of bromelain, use sodium hydroxide and sulfuric acid to adjust the pH value, and control the pH value of the reaction system to 7.5, and the enzymatic hydrolysis temperature is 60°C. The solution time is 5h. After the enzymatic hydrolysis reaction, use a centrifuge with a rotating speed of 10,000 rpm to remove solid impurities, and use a nanofiltration membrane with a molecular weight cut-off of 3 kD to remove foreign proteins from the obtained supernatant to obtain a shark chondroitin sulfate solution.

[0042] 3. Put the above-mentioned shark chondroitin sulfate solution in an ultrasonic reaction tank, add zinc...

Embodiment 3

[0047] A preparation method of shark chondroitin sulfate chelated zinc salt, comprising the following steps:

[0048] 1. Shark cartilage removes fat, shark meat, and calcified bones. After pulverizing with a pulverizer, 10kg of shark cartilage powder is obtained.

[0049] 2. Put the above-mentioned shark cartilage powder in a reaction kettle, add 100kg of water, add 250g of bromelain and 250g of neutral protease respectively, use sodium hydroxide and sulfuric acid to adjust the pH value, and control the pH value of the reaction system to 7, enzymolysis The temperature is 50°C, and the enzymatic hydrolysis time is 3h. After the enzymatic hydrolysis reaction, use a centrifuge with a rotating speed of 16,000 rpm to remove solid impurities, and use a nanofiltration membrane with a molecular weight cut-off of 1 kD to remove foreign proteins from the obtained supernatant to obtain a shark chondroitin sulfate solution.

[0050] 3. Put the above-mentioned shark chondroitin sulfate so...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Relative molecular massaaaaaaaaaa
Average relative molecular weightaaaaaaaaaa
Relative molecular massaaaaaaaaaa
Login to view more

Abstract

The invention relates to a preparation method of shark chondroitin sulfate chelated zinc salt. The method comprises the following steps: pretreating shark cartilage to obtain shark cartilage powder, and preparing shark chondroitin sulfate by utilizing an enzyme extraction and centrifugal machine separating synergistic nanofiltrating membrane purification linkage technology. The shark chondroitin sulfate zinc salt crude product is prepared by taking the shark chondroitin sulfate as a raw material and utilizing an ultrasonic cavitation and shark chondroitin sulfate zinc chelation technology. And a shark chondroitin sulfate zinc salt powder finished product is prepared by utilizing a centrifugal separating electrodialysis purification and spray drying integrated technology. The method is mild in reaction condition, controllable in quality and suitable for large-scale production. The shark chondroitin sulfate zinc salt prepared by the preparation method disclosed by the invention is concentrated in average relative molecular mass, good in solubility, high in bioavailability, high in safety and free from toxic and side effects, and can be applied to the fields of antiinflammatory health foods and medicines.

Description

technical field [0001] The invention relates to the field of deep processing of aquatic products and belongs to the field of biotechnology, in particular to a preparation method and application of shark chondroitin sulfate chelated zinc salt. Background technique [0002] Chondroitin Sulfate (CS) is an acidic polymeric viscous polysaccharide from animal cartilage tissue. The sugar chain of CS is composed of alternating glucuronic acid and N-acetylgalactosamine disaccharide units. Its biological structure determines its It has the biological activity of regulating bone and joint function. In European and American countries, CS and its derivatives are widely used in the prevention and treatment of arthritis. In my country, in 2010, the Pharmacopoeia of the People's Republic of China listed CS as a routine drug for the prevention and treatment of joint diseases. my country is the largest country in CS production. There are about 200 domestic manufacturers, and the output acco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/08A23L33/125A23L33/165A61K33/30A61K31/737A61P29/00
CPCC08B37/0069C08B37/0003A23L33/125A23L33/165A61K33/30A61K31/737A61P29/00A23V2002/00A61K2300/00A23V2250/1642
Inventor 陈俊德高然
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap