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Lactic acid bacteria starter containing protein fibers and preparation method thereof

A technology of lactic acid bacteria fermentation and protein fiber, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., to achieve the effects of wide source, good storage stability and low price

Pending Publication Date: 2022-01-04
HUBEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Protein fibers have the characteristics of high stiffness, extreme aspect ratio, and collective order, which can promote different interactions with various nutrients, and the functional groups widely present on the surface have good antioxidant properties, hydrophobicity, emulsification, and foaming properties. The formation of protein fibers further expands, broadens and enriches the functions of proteins. However, there are no reports on the application of protein fibers to microbial starters, especially lactic acid bacteria starters.

Method used

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  • Lactic acid bacteria starter containing protein fibers and preparation method thereof
  • Lactic acid bacteria starter containing protein fibers and preparation method thereof
  • Lactic acid bacteria starter containing protein fibers and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment provides a lactic acid bacteria starter prepared by utilizing whey protein isolate fiber and its preparation method, specifically as follows:

[0025] Step 1. Inoculate the frozen-preserved Lactobacillus reuteri TMW 1.656 (derived from the Food Microbiology Laboratory of the University of Alberta) into mMRS liquid medium for activation, and culture it statically at 37° C. for 24 hours; then add 4% (v / v) The inoculum was transferred to a new 100mL mMRS liquid medium, and cultured at 37°C for 24 hours; refrigerated and centrifuged at 4°C and 10,000rpm for 15 minutes, the bacteria were collected, washed twice with peptone solution, and re- Suspended in 5mL peptone aqueous solution to make a concentration of 10 9 CFU / mL bacterial suspension, for later use;

[0026] Step 2, taking a certain amount of whey protein isolate powder and dissolving it in sterile water, fully dissolving it, and preparing a whey protein isolate solution with a concentration of 3% (w...

Embodiment 2

[0032] The difference between this example and Example 1 is that in step 3, the whey protein isolate fiber with a concentration of 4% (w / w) is prepared.

Embodiment 3

[0034] The difference between this example and Example 1 is that in step 3, the whey protein isolate fiber with a concentration of 5% (w / w) is prepared.

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Abstract

The invention discloses a lactic acid bacteria starter containing protein fibers and a preparation method thereof. The lactic acid bacteria starter is prepared from lactic acid bacteria and the protein fibers. Based on the oxidation resistance and hydrophobicity of the protein fibers and the mutual attraction effect of the protein fibers and lactic acid bacteria cells, the powdery lactic acid bacteria starter can be obtained through simple physical blending and drying, the concentration, the pH value and the like of the protein fibers are optimized, the storage survival rate of the lactic acid bacteria is remarkably increased, and the shelf life of the lactic acid bacteria starter is prolonged; and compared with a lactic acid bacteria starter prepared from common protein, the lactic acid bacteria starter prepared from the protein fiber has better storage stability. Meanwhile, the lactic acid bacteria starter containing the protein fibers is wide in raw material source and low in price; and the preparation method is simple to operate, short in preparation time, easy for industrial production and extremely high in production and application value.

Description

technical field [0001] The invention belongs to the technical field of microbial starters, and in particular relates to a lactic acid bacteria starter containing protein fibers and a preparation method thereof. Background technique [0002] The application of microbial starter as a new type of biological product in food is no longer unfamiliar. In recent years, it has become more and more common in dairy products, meat products, fish products, etc. However, the activity of microbial starters in storage is affected by factors such as oxygen, temperature, and moisture. High water activity, storage temperature, and the presence of oxygen will accelerate the death of microorganisms, shorten the shelf life of products, and limit the diversification of microbial starters. . In order to facilitate customers and reduce the cost of manufacturers, it is of great significance to realize the long-term storage of dry microbial starter. The main methods to prolong the shelf life of star...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04C12R1/225
CPCC12N1/20C12N1/04
Inventor 赵萌郭前婉
Owner HUBEI UNIV OF TECH
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