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Lysozyme composition with anti-inflammatory effect

A technology of lysozyme and composition, applied in the field of lysozyme composition, can solve the problem that the anti-inflammatory effect needs to be improved, and achieve the effects of excellent anti-inflammatory performance, natural origin, and no toxic and side effects

Active Publication Date: 2022-01-07
浙江莱康生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the anti-inflammatory effect of lysozyme prepared by fermentation process needs to be improved, and there is an urgent need for a lysozyme composition prepared by fermentation process with good anti-inflammatory effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, fermentation process prepares lysozyme L1 (Streptomyces albicans LA-7)

[0032] Take 30mL of liquid medium A (containing 1% yeast extract powder, 2% tryptone and 2% glucose in water) and place it in a conical flask, sterilize and cool to 30°C, and inoculate according to the inoculum size of 5% v / v Inject the thalline seed of Streptomyces albicans LA-7; continuously cultivate in a constant temperature shaker at 30°C and 250r / min for 24h; take 1mL of the seed liquid cultivated in the above steps and inoculate it into 100mL sterilized liquid medium B (containing 1 % yeast extract powder, 2% tryptone, 1% amino acid-free yeast nitrogen source, 0.00004% biotin and 1% glycerin water) conical flask, at 30 ℃, 250r / min constant temperature shaker for 24h The fermented liquid cultivated in the above steps is micro-filtered through a ceramic membrane, and the filtrate is obtained by ultrafiltration to concentrate components with a cut-off molecular weight of 10-30kDa ...

Embodiment 2

[0033] Embodiment 2, fermentation process prepares lysozyme L2 (Bacillus subtilis YT-25)

[0034] Take 30mL of liquid medium A (containing 1% yeast extract powder, 2% tryptone and 2% glucose in water) and place it in a conical flask, sterilize and cool to 30°C, and inoculate according to the inoculum size of 5% v / v Into the thalline seeds of Bacillus subtilis YT-25; at 30 ℃, 250r / min constant temperature shaker in continuous culture for 24h; take 1mL of the seed liquid cultivated in the above steps and inoculate it into 100mL sterile liquid medium B (containing 1 % yeast extract powder, 2% tryptone, 1% amino acid-free yeast nitrogen source, 0.00004% biotin and 1% glycerin water) conical flask, at 30 ℃, 250r / min constant temperature shaker for 24h The fermented liquid cultivated in the above steps is micro-filtered through a ceramic membrane, and the filtrate is obtained by ultrafiltration to concentrate components with a cut-off molecular weight of 10-30kDa to obtain a concent...

Embodiment 3

[0035] Embodiment 3, fermentation process prepares lysozyme L3 (Streptomyces albicans LA-7+Bacillus subtilis YT-25)

[0036]Take 30mL of liquid medium A (containing 1% yeast extract powder, 2% tryptone and 2% glucose in water) and place it in a conical flask, sterilize and cool to 30°C, and inoculate according to the inoculum size of 2.5% v / v Insert the thalline seeds of Bacillus subtilis YT-25, and insert the thalline seeds of Streptomyces albicans LA-7 at the same time according to the inoculation amount of 2.5% v / v; continuously cultivate in a constant temperature shaker at 30°C and 250r / min for 24h Get 1mL of the seed liquid cultivated in the above steps and inoculate it into 100mL sterilized liquid medium B (containing 1% yeast extract powder, 2% tryptone, 1% amino-free yeast nitrogen source, 0.00004% biotin and 1% glycerol) Aqueous solution) in an Erlenmeyer flask, at 30°C, 250r / min constant temperature shaker for continuous culture for 24h; the fermentation broth cultiv...

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Abstract

The invention relates to a lysozyme composition with an anti-inflammatory effect. The active ingredient lysozyme in the lysozyme composition is prepared from thallus seeds of streptomyces albus and / or bacillus subtilis by adopting a fermentation process. The lysozyme is natural in source and free of toxic and side effects, and the preparation method of the lysozyme composition can be industrialized and has popularization and application value.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a lysozyme composition. Background technique [0002] Lysozyme, also known as cell wall lytic enzyme, is an alkaline proteolytic enzyme that can hydrolyze and stabilize cell walls. It mainly acts on the bacterial cell wall, that is, it acts on the β-1,4 glycosidic bond between N-acetylglucosamine and N-acetylmuramic acid in its peptidoglycan, and promotes the polysaccharide in the bacterial cell wall by hydrolyzing it. It decomposes into glycopeptides, reduces the stability of the bacterial cell wall, destroys its structure, and finally causes the cell wall to lyse the contents of the cell to flow out of the cell, causing the bacteria to dissolve. Since lysozyme is a protein with the above-mentioned bacteriolytic effect and has no toxic side effects on the human body, it can also be used in the food industry to make preservative materials. Lysozyme is a non-specific immune factor prese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/36C12N1/20A61K38/47A61P29/00C12R1/125C12R1/47
CPCC12N9/2462C12N1/20C12Y302/01017A61K38/47A61P29/00Y02A50/30
Inventor 陈生红
Owner 浙江莱康生物工程有限公司
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