Fusarium fujikuroi for preventing and controlling monochamus alternatus hope and application of fusarium fujikuroi
A technology of Fusarium fusarium and longhorn beetle, applied in the directions of application, fungi, microorganism-based methods, etc., can solve the problem of Fusarium fusarium that has not been seen to prevent and control the longhorn beetle, and achieves easy fermentation production, Significant insecticidal activity, reduced transmission effect
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Embodiment 1
[0017] Example 1: Isolation and Identification of Bacterial Strains
[0018] 1. Isolation of Strain CS-12
[0019] Different kinds of beetles were collected from Wulian Mountain, Jiuxian Mountain, Laoshan Mountain in Qingdao, and Mount Tai'an in Rizhao, Shandong Province. The fungus was isolated by dilution coating method on Bengal red medium. The specific method is as follows:
[0020] (1) Sterilization: Autoclave for 25 minutes at 0.1MPa and 121°C for the experimental supplies used, such as petri dishes, pipette tips, and sterile water, and set aside;
[0021] (2) Disinfection: Rinse the body surface of longicorn beetle adult 3 times with 75% ethanol, each time for 2 minutes, then rinse 3 times with sterile water, and grind after natural drying;
[0022] (3) Grinding: Weigh 5 g of sterilized longicorn beetle into a sterile centrifuge tube, add 5 mL of sterile water, and grind thoroughly with a grinding rod.
[0023] (4) Gradient dilution: transfer all the ground juice to ...
Embodiment 2
[0042] Embodiment 2: Fusarium Fujikura CS-12 fermentation broth preparation
[0043] (1) Activate and inoculate Fusarium Fujikura CS-12 on the surface of a PDA plate, and incubate at a constant temperature of 28°C for 72h;
[0044] (2) punch the bacteria cake of the above-mentioned activated bacterial strain, inoculate it in the seed liquid culture medium, and cultivate it in a shaker flask at 28° C. and 130 r / min for 72 hours to obtain the seed liquid of Fusarium Fujikura CS-12;
[0045] The seed liquid culture medium is: by mass percentage, glucose 4%, peptone 1%, yeast powder 1%, the rest is water, and the pH is adjusted to 7.0-7.2.
[0046] (3) the seed liquid prepared by step (2) is inoculated in the fermentation medium, the inoculum size is 1:100, and the fermentation condition and the fermentation medium are the same as step (2), to obtain the fermentation liquid of Fusarium Fujikura CS-12, and adjusted to 5×10 8 CFU mL -1 concentration.
[0047] The fermentation me...
Embodiment 3
[0048] Embodiment 3: Determination of anti-insect activity of Fusarium Fujikura CS-12
[0049] (1) Experimental method
[0050] First prepare fermented liquid containing different concentrations of Fusarium Fujikura CS-12: the concentration of Fusarium Fujikura CS-12 fermented liquid prepared in Example 2 is adjusted to 0.2×10 8 CFU mL -1 , 0.4×10 8 CFU mL -1 , 0.8×10 8 CFU mL -1 , 1.6×10 8 CFU mL -1 and 3.2×10 8 CFU mL -1 .
[0051] Soak the collected monochamus alternatus (adult, body length about 20 mm, and the feeding conditions are consistent to ensure that the vitality is basically the same) in the suspension of Fusarium fujikura CS-12 containing different concentrations of spores for 5 seconds, and then transferred to the container. Observation was carried out in a clean rearing box with black pine needles of that year, and 15 adults were inoculated for each treatment. Each treatment was repeated three times, and the sterilized fermentation medium was used as...
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