Lactobacillus plantarum for regulating OVA specific IgE and application of lactobacillus plantarum
A technology of Lactobacillus plantarum and microbial strains, applied in the field of microbiology and medicine, can solve the problems that can not be cured fundamentally, can not change the pathological damage of intestinal tissue, and achieve the effect of alleviating allergic reactions
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Embodiment 1
[0086] Embodiment 1: the acquisition of Lactobacillus plantarum (Lactobacillus plantarum) CCFM1189
[0087] (1) Lactobacillus plantarum (Lactobacillus plantarum) CCFM1189 screening:
[0088] Use a pipette gun to draw a sample of the fermented fruit and vegetable fermentation broth (sample number is VJLHD12), use physiological saline to carry out gradient dilution of the sample, take 100 μL of the diluted solution to coat the plate (LBS solid medium, add 0.5‰ medium volume before pouring the plate) Sterile nystatin), placed in a 37°C constant temperature incubator and cultured upside down for 48 hours, classified according to the colony morphology, picked a single colony and streaked it on the LBS solid medium (the strain CCFM1189 was the fourth colony picked ), placed in a 37°C constant temperature incubator and cultured upside down for 48 hours, picked a single colony and inoculated it into 5 mL of liquid LBS medium, and placed it in a 37°C constant temperature incubator for ...
Embodiment 2
[0092] Embodiment 2: the cultivation of plant lactobacillus (Lactobacillus plantarum) CCFM1189
[0093] Specific steps are as follows:
[0094] Insert Lactobacillus plantarum CCFM1189 and Lactobacillus plantarum L.plantarum 1 into MRS liquid medium and culture at 37°C for 16 hours, then absorb the bacterial liquid and transfer it to fresh MRS liquid medium according to the inoculation amount of 4%, and cultivate under the same conditions for 12 hours , 8000r / min centrifuged thallus for 15min, washed thalline with 0.9% normal saline and then centrifuged again at 8000r / min for 10min, collected the thalline, resuspended with 30% (m / v) sucrose solution, prepared respectively to obtain the resuspension, Freeze at -80°C until use.
[0095] Preparation of bacterial suspension for gavage: When Lactobacillus plantarum is used for gavage to mice, take out the prepared suspension from -80°C, centrifuge at 4°C, 8000r / min for 10min, discard clear, and resuspended with 0.9% normal saline ...
Embodiment 3
[0096] Embodiment 3: Alleviating effect of plant Lactobacillus (Lactobacillus plantarum) CCFM1189 on weight loss of food allergic mice
[0097] Specific steps are as follows:
[0098] The 4-5 week-old SPF BALB / c female mice were divided into 4 groups, namely the normal group, the model group and the experimental group. L. plantarum 1 group of L. plantarum 1;
[0099] 6 animals in each group were raised in the Experimental Animal Center of Jiangnan University, fed with common feed, with a constant temperature of 21-26°C, humidity of 40-70%, noise less than or equal to 60dB, and animal illumination of 15-20LX (all animal experiment procedures were provided by Jiangnan University Animals). Welfare and Ethics Committee review and approval).
[0100] The experimental period was 7 weeks in total, and the first week was the adaptation period. After the adaptation period, the mice were weighed. During the adaptation period, they were fed: Co60 irradiated experimental mouse growth an...
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